Usha Nandhini Devi Harinarayanan¹*, Karthikeyan Subburamu¹, Geetha Padmanaban¹, Swetha Balakrishnan², Subhavyuktha Sadasivan² and Gayathri Muthusamy³

¹Centre for Post Harvest Technology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India
²Department of Vegetable Science, Horticultural College and Research Institute, TamilNadu Agricultural University, Coimbatore, Tamil Nadu, India
³Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India

*Corresponding Author: Usha Nandhini Devi Harinarayanan, Centre for Post Harvest Technology, TamilNadu Agricultural University, Tamil Nadu, India.

Received: August 08, 2025; Published: August 26, 2025

Abstract

Indian spinach, Basella rubra L. has been traditionally used in Indian medicine to treat specific diseases. The present investigation was carried out to examine the antimicrobial activity of the fruit extracts of Basella rubra fruits by measuring the zones of inhibition using Disc Diffusion method and Agar well Diffusion method against three species of microorganisms viz., Bacillus subtilus, Escherichia coli and Saccharomyces species. The fruit extract of Basella was dissolved in methanol to obtain different concentrations (25 mg/ml, 50 mg/ml and 100 mg/ml). 0.5ml of methanol was used as negative control (solvent control). The extract showed significant antimicrobial activity against Bacillus subtilus, Escherichia coli and Saccharomyces species and the inhibition zones were significantly different in each dilution of the fruit extract. The minimum inhibitory concentration of the extract against the different strains was found to be 25mg/ml.

Keywords: Basella Rubra; Antimicrobial Activity; Disc Diffusion Method; Agar Well Diffusion Method; Inhibitory Concentration

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Citation

Citation: Nora M Ezz El-Ragal., et al. “Quality Characteristics of Pan bread Fortified by Moringa oleifera Powder of Leaves and Seeds".Acta Scientific Nutritional Health 9.9 (2025): 46-50.

Copyright

Copyright: © 2025 Nora M Ezz El-Ragal., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.