Peter Uwadiegwu Achukwu1, Richard Ikechukwu Eze1 and Nosa Terry Omorodion2*
1Department of Medical Laboratory sciences, Faculty of Health Sciences and Technology, College of medicine, University of Nigeria, Enugu
2Health Services Department, University of Benin, Benin city, Edo state, Nigeria
*Corresponding Author: Nosa Terry Omorodion, Health Services Department, University of Benin, Benin city, Edo state, Nigeria.
Received: May 16, 2018; Published: July 02, 2018
Citation: Nosa Terry Omorodion., et al. “Rapid Method Estimation of Bromate in Serum and Bread Consumed in Enugu, Nigeria". Acta Scientific Nutritional Health 2.8 (2018).
The aim of this study is to formulate rapid method for detection and estimation of bromate in serum and bread consumed in Enugu, Nigeria. All bread samples tested positive by the quantitative method, showing the presence of black spots. The black spot did not fade even after 24 hours. The filtrate of the same bread samples when applied on Whatman No 1 filter paper before dropping the potassium iodide reagent gave no black spot. Similarly, when the reagent dropped directly negative results negative results were obtained. The same samples were also tested using flooding method, described in the promethazine hydrochloride method and similar results were obtained. However, the colour for promethazine hydrochloride method was pink. The potassium iodide reagent gave consistently positive results throughout its storage period of three (3) months when applied directly on the loaf of bread. The methylene blue reagent developed for quantitative test was also tested using the quantitative procedure. The result showed the disappearance of the blue colour to the background colour almost similar to that of the bread sample. The analytical performance was measured by determining eight (8) out of ten (10) of the positive sample were reported positive while four (4) out of five (5) negative samples were reported negative. This implies a false negative of two (2) and false positive of one (1). Apart from the interference of extraneous factors, whose presence did not interfere with the positivity and sensitivity of the spot and semi-quantitative, the quantitative procedure may be applied in bromate estimation in bread with caution.
Keywords: Bromate; Estimation; Confectionaries; Formulation
Copyright: © 2018 Nosa Terry Omorodion., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.