R Batista-Ramos¹, L García-Delgado⁴, S Mireles-Flores⁴, C Olmo-González⁴, I Aguilera-Garces², B López-Valoy2, D Verdecia-Acosta², C Lemus-Flores³ and F Grageola-Nuñez³

¹CCS José Martí Pérez, Sabanilla Barranca, Bayamo, Cuba
²Universidad de Granma-Carretera de Manazanillo Granma, Cuba
³Universidad Autónoma de Nayarit, Mexico
⁴Centro Universitario de Ciencias Biológico y Agropecuarias (CUCBA), Universidad de Guadalajara, Camino del Astillero, Zapopan, Jalisco, México

*Corresponding Author: F Grageola-Nuñez, Universidad Autónoma de Nayarit, Mexico.

Received: August 29, 2025; Published: September 17, 2025

Abstract

In order to evaluate the inclusion of Moringa oleifera foliage meal (HFMO) in free-range layer diets and its impact on bio-productive indicators, a study was developed that considered 200 30-week-old k53 line hens housed in 2 m2 quarters (5 birds/m2), and distributed in a completely randomized design with five replications, with inclusion levels (0, 6, 8 and 10 %) being considered as treatments. The following variables were considered: Viability (%); Feed intake (kg); Live weight/bird (g); egg/bird (U); laying intensity (%); broken eggs (U), cracked eggs (U) and dirty eggs (U). To determine the indicators of external egg quality, the following variables were considered: egg weight (g), width (cm), egg equator with a caliper and egg length (cm) between the poles, shell thickness, porosity, and for internal quality, the variables height, yolk diameter and white (mm). Data were compared using the Duncan Twelfth for the comparison of means at a 95% confidence level. The consumption of HFMO showed differences between treatments (P≤0.05), increasing by 15.60 g/bird/day. In the live weight of the free-range layers there were no differences for p≥0.05; in initial measurements (2,200 and 2,198 g) at the end of the first week of evaluation; with discrete differences (2,202 and 2,199 g) after the experiment. Concluding that up to 8% showed the best results in the diet of layers without affecting bioproductive indicators; however, employment up to 10% is possible for non-specialized breeding.

Keywords: Alternative Feed; Productive Quality; Free-Range Layers

References

1. Abd El-Hack., et al. “Pharmacological, nutritional and antimicrobial uses of Moringa oleifera leaves in poultry nutrition: an up-to-date knowledge”. Poultry Science 9 (2022): 102031.
2. Ahmad S., et al. “Effect of Moringa oleifera (Lam.) pods as a food additive on egg antioxidants, chemical composition and performance of commercial coatings”. South African Journal of Animal Science6 (2017): 864-874.
3. Ascanio JCB. “Study of the effects of the implementation of Moringa (Oleifera) on poultry production”. Francisco de Paula Santander (2021):
4. Bartlett MS. “Properties of Sufficiency and Statistical Tests. Proceedings of the Royal Society of London”. Series A, Mathematical and Physical Sciences 901 (1937): 268-282.  
5. Bidura IGNG., et al. “Effect of Moringa oleifera leaf powder in diets on laying hens performance, β-carotene, cholesterol, and minerals contents in egg yolk. International Seminar on Chemical Engineering Soehadi Reksowardojo (STKSR)”. Materials Science and Engineering (2020).
6. Brunetti L., et al. “By-products of the food industry as a raw material for the formulation of innovative organic feed: a sustainable alternative for poultry feed”. Molecules15 (2022): 4735.
7. Duncan DB. “Multiple Range and Multiple F Tests”. Biometrics1 (1955): 1-42.
8. “The impact of the war in Ukraine on global nutrition and food security. Food and Agriculture Organization of the United Nations”. Rome (2023).
9. Fernández-Ruelas EP., et al. “Productive response of the inclusion of moringa (moringa oleifera) in the feed of hens in the second production period”. Ariotake - Journal of Veterinary Research and Amazonia 2 (2022): e187.
10. Fumero JE., et al. “Technological manual for poultry breeding. Semi-rustic breeding stock and their replacements. Free-range breeding stock. IIA Havana, Cuba. MINAG. UECAN (Union of Companies of the National Poultry Combine) (2011).
11. Gayathri L., et al. “Effect of Dietary Supplementation of Moringa oleifera Leaf Meal on Production Performance and Egg Quality of Vanaraja Laying Hens”. ANIMAL NUTRITION AND FEED TECHNOLOGY 2 (2020): 279-287.
12. Govoni C., et al. “Global assessment of natural resources for chicken production”. Advances in Water Resources 154 (2021): 103987.
13. Guerrero Díaz SM., et al. “Evaluation of the internal and external quality and shelf life of commercial eggs under tropical conditions”. Spei Domus2 (2023): 1-15.
14. “Time, Current Time. Institute of Meteorology of the Republic of Cuba
15. Jin Y and Carrillo M. “Lohmann guide for handling laying cage systems (2019).j
16. Khan UK., et al. “Possible applications of Moringa oleifera in poultry health and production as an alternative to antibiotics: a review”. Antibiotics 12 (2021): 1540.
17. Ledea-Rodríguez JL., et al. “Seed production of Moringa oleifera Lam varieties according to planting density and successive harvests”. Agronomía Mesoamericana1 (2022): 50528.
18. Lilliefors HW. “On the Kolmogorov-Smirnov test for normality with mean and variance unknown”. Journal of the American Statistical Association318 (1967): 399-402.
19. Lu W., et al. “Evaluation of Moringa oleifera leaf in laying hens: Effects on laying performance, egg quality, plasma biochemistry and organ histiophatological indices”. Italian Journal of Animal Science 15 (2016): 658-665.
20. Mabusela SP., et al. “Layer performance, fatty acid profile and the quality of eggs from hens supplemented with Moringa oleifera whole seed meal”. South African Journal of Animal Science (2018).
21. Manman Shen., et al. “Effect of dietary inclusion of Moringa oleifera leaf on productive performance, egg quality, antioxidant capacity and lipid levels in laying chickens”. Italian Journal of Animal Science 20 (2021): 2012-2021.
22. Martínez-Gutiérrez MS., et al. “Proposal of protein plants in the feeding of the Animal Genetic Resources (RZG) of birds in Cuba”. Revista Cubana de Ciencia Avícola2 (2017): 77-83.
23. Narushin V. “Production, Modeling, and Education Egg Geometry Calculation using the Measurements of Length and Breadth”. Poultry Science 84 (2005): 482-484.
24. Narushin VG., et al. “A novel Egg Quality Index as an alternative to Haugh unit score”. Journal of Food Engineering 289 (2021): 110176.
25. Nguyen Tuyet Giang., et al. “Moringa leaf powder as natural feed additive on productive performance and egg quality of ISA Brown laying hens”. Livestock Research for Rural Development (2023).
26. OECD-FAO. “Agricultural Outlook 2021-2030. Organization for Economic Cooperation and Development and Food and Agriculture Organization of the United Nations (2021).
27. Pampín M., et al. “Contribution to the knowledge of the productive indicators of the semi-rustic hen in the stage of reproduction”. Revista Cubana de Ciencia Avícola1 (2017): 61-66.
28. Pliego AB., et al. “Beneficial and adverse effects of medicinal plants as food supplements in poultry nutrition: a review”. Animal Biotechnology 2 (2022): 369-391.
29. Rajesh P., et al. “Dietary inclusion of Moringa oleifera leaf meal with enzyme supplementation on egg production and egg quality of laying hens”. Indian Journal of Animal Nutrition 1 (2022): 88-96.
30. Rocha Limón F. “Moringa oleifera meal in the feed laying birds. Agropecuaria-Universidad Evangélica Boliviana”. Science and Culture - Research and Dissemination of Services (2020).
31. Romera BM., et al. “Dynamic behavior of egg weight in free-range hens discriminated by productive indicators at sexual maturity”. Ciencia Veterinaria 2 (2020): 71-96.
32. Romero-Yerena., et al. “Effect of the addition of Moringa (Moringa Oleifera) in the diet of semi-heavy hens on post-pellet recovery and egg quality”. Tropical and Subtropical Agroecosystems 25 (2022): 070.
33. Ruelas EPF., et al. “Addition of moringa leaf meal in the diet of hy-line brown laying hens: influence on productive and egg quality parameters”. Journal of Animal Health and Production 2 (2023): 185-192.
34. Rugel DO and Emén MF. “Inclusion of Moringa oleifera meal in diets for broilers”. Revista Veterinaria Argentina 1 (2020): 74-77.
35. Sánchez Flores D and Barreto L. “Moringa oleifera flour as a feed alternative for laying hens of the Babcock Brown line in the village of El Carbonal in the municipality of Rio de Oro. School of Agricultural, Livestock and Environmental Sciences - ECAPMA Zootechnics (2024).
36. Santos WDD and Silva AF. “Sustainability of poultry production in the state of Goiás: An analysis from the perspective of the New Institutional Economics”. Revista Latinoamericana de Ambiente Construído and Sustentabilidade15 (2023).
37. Scull-Rodríguez I., et al. “Evaluation of secondary metabolites in Stizolobium aterrimum (mucuna) forage meal, for use in animal feed”. Pastures and Forages 40.4 (2017): 302-307.
38. Shad M and Xiang SP. “Application of Moringa (Moringa oleifera) as natural feed supplement in poultry diets”. Animals 9 (2019): 1-19.
39. Su B and Chen X. “Current and potential status of Moringa oleifera leaf as an alternative source of protein for animal feed”. Frontiers in Veterinary Science 7 (2020): 53.
40. Teteh A., et al. “Effects of Moringa oleifera Leaf on Laying Rate, Egg Quality and Blood Parameters”. International Journal of Poultry Science7 (2016): 277-282.
41. Toalombo PA., et al. “Productive and organoleptic characterization of eggs from free-range hens from the highlands region of Ecuador”. Archives of Zootechnics 263 (2019): 412-415.
42. Doc. 6. Practical system for feed quality care between poultry and feed companies. Sections 6, 7 and 8. Havana, MINAG (2015).
43. Valdivié M., et al. “Use of diets with Moringa oleifera (stems + leaves) meals in laying hens”. Revista Cubana de Ciencia Agrícola3 (2016): 445-454.
44. Windhorst HW. “PROJECTION OF GLOBAL EGG PRODUCTION UNTIL” 2030 (2022):

Citation

Citation: F Grageola-Nuñez.,et al. “Inclusion of Foliage Meal of Moringa oleifera Lamark in Free-Range Layer Diets. Impact on Bioproductive Behaviour".Acta Scientific Veterinary Sciences 7.10 (2025): 30-39.

Copyright

Copyright: © 2025 F Grageola-Nuñez., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.