Sanmeet Kour¹, Deepti Narang²*, Mudit Chandra², Sikh Tejinder Singh², Ashwani Kumar Sharma³, Ramneek⁴ and Kuldip Gupta³

¹Ph.d Research Scholar, Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
²Principal Scientist, Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
³Professor, Department of Veterinary Medicine, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
⁴Professor, Animal Disease Research Centre, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India

*Corresponding Author: Deepti Narang, Principal Scientist, Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India.

Received: July 18, 2025; Published: August 26, 2025

Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne's disease, a chronic wasting condition that affects ruminants and causes intestinal inflammation and intermittent diarrhea. The immune response to Mycobacterium avium subspecies paratuberculosis is mostly dependent on cellular immunity, and Th1 cell-produced cytokines are particularly crucial. Early infections with Mycobacterium avium subspecies paratuberculosis (MAP) appear to be frequently accompanied by the production of pro-inflammatory cytokines like IFN- γ and TNF-α by Th1-type T cells. The present study was conducted to analyze the cytokine gene expression of JD positive animals which were detected positive by IS900 -PCR and were compared to five healthy animals. The gene expression of IFN- γ was found to be more upregulated in animals than TNF- α as increased production of IFN- γ is characteristic of the infection caused by Mycobacterium as it is considered critical for control of mycobacterial infections.

Keywords: MAP; Cytokines; TNF- α, IFN- γ; IS900 PCR

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Citation

Citation: Deepti Narang., et al. “Cytokine Gene Expression of IFN-γ and TNF-α in Animals Infected with Johnes Disease".Acta Scientific Veterinary Sciences 7.9 (2025): 23-29.

Copyright

Copyright: © 2025 Deepti Narang., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.