Arjuma Khatun and Nafis I Assad*

Division of Animal Reproduction Gynaecology and Obstetrics, FVSc & AH, Shuhama, SKUAST-Kashmir-190006 (J&K), India

*Corresponding Author: Nafis I Assad, Division of Animal Reproduction Gynaecology and Obstetrics, FVSc & AH, Shuhama, SKUAST-Kashmir-190006 (J&K), India.

Received: May 19, 2025; Published: June 20, 2025

Abstract

Bovine serum Albumin (BSA) is the most abundant plasma protein found in animals that is primarily associated with transport of biologically active components via systemic circulation to the target cells. Foetal Bovine Serum (FBS) is a combination product of various sugars, lipids, amino acids, vitamins, growth factors, trace elements and hormones. It is used for growth and maintenance of most types of cell and tissue cultures. Both BSA and FBS are more advantageous in terms of affordability, availability, ease of handling as well as comprehensive quality control checks and assurance testing than the existing extender additives like egg-yolk, coconut milk or skimmed milk. In order to tap the advantages of these proprietary preparations both BSA and FBS have been used in semen preservation. It was found that BSA can inhibit lipid peroxidation in sperm membrane besides improving motility and viability post freezing. It was also found that BSA can substitute egg yolk in the semen extenders of some animals. FBS has been used in semen cryopreservation to take advantage of its buffering properties and benefit from its proteins that confer mechanical protection to plasma membrane. It was further documented that addition of FBS reduced malondialdehyde production (MDA) and raised glutathione peroxidase activity (GPx) in the extended Semen.

Keywords: Mast Cell Tumour; African Wild Dog; Neoplasms

References

1. Evans G and MaxwelL WMC. “Frozen storage of semen”. In: Salamon’s Artificial Insemination of Sheep and Goats. Wellington: Butterworths (1987).
2. Mahdi SA., et al. “Effect of different concentrations of bovine serum albumin on some of the frozen sperm characteristics of the rams”. Plant Archives2 (2019): 1486-1488.
3. Batellier F., et al. “Advances in cooled semen technology”. Animal Reproduction Science3-4 (2001): 181-190.
4. Frank WP. “The Plasma Proteins: Structure, Function and Genetic Control”. 2^nd, Academic Press, New York 1 (1975): 141-147.
5. Uysal O., et al. “Effect of bovine serum albumin on freezing of canine semen”. Indian Veterinary Journal 82 (2005): 97-98.
6. J van der Valk., et al. “Optimization of chemically defined cell-culture media - replacing foetal bovine serum in mammalian in vitro methods”. Toxicology Vitro 24 (2010): 1053-1063.
7. M Verdanova., et al. “Evaluation of sericin as a fetal bovine serum-replacing cryoprotectant during freezing of human mesenchymal stromal cells and human osteoblast-like cells”. Biopreservation and Biobanking 12 (2014): 99-105.
8. Blank MH., et al. “Beneficial influence of foetal bovine serum on in vitro cryo-survival of chicken spermatozoa”. Cryobiology 95 (2020): 103-109.
9. Nair S J., et al. “A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature”. Animal Reproduction Science 96 (2006): 21-29.
10. Baumber J., et al. “Assessment of the cryopreservation of equine spermatozoa in the presence of enzyme scavengers and antioxidants”. American Journal of Veterinary Research 66 (2005): 772-779.
11. Sarıozkan S., et al. “The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen”. Cryobiology 67 (2013): 1-6.
12. Kreider J L., et al. “Inclusion of bovine serum albumin in semen extenders to enhance maintenance of stallion sperm viability”. Theriogenology 23 (1985): 399-408.
13. Matsuoka T., et al. “Effects of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa”. Journal of Reproduction and Development 52 (2006): 675-683.
14. Bakst MR and Cecil HC. “Effect of bovine serum albumin on motility and fecundity of turkey spermatozoa before and after storage”. Journal of Reproduction and Infertility 94 (1992): 287-293.
15. Cabrita E., et al. “Effect of external cryoprotectants as membrane stabilizers on cryopreserved rainbow trout sperm”. Theriogenology 56 (2001): 623-635.
16. Saleh Abed Al Wahed Mahdi., et al. “Effect of different concentrations of bovine serum albumin on some of the frozen sperm characteristics of the rams”. Plant Archives2 (2019): 1486-1488.
17. Kaewkesa T., et al. “The goat semen quality after being frozen using albumin and cholesterol substituted for egg yolk in semen extender”. The Thai Journal of Veterinary Medicine 46 (2016): 201.
18. Zhang XG., et al. “Effects of bovine serum albumin on boar sperm quality during liquid storage at 17°C”. Reproduction in Domestic Animals2 (2015): 263-269.
19. Alomar M. “Influences of bovine serum albumin on fresh and chilled ram sperm motility assessed by computer aided sperm analyser”. Bulgarian Journal of Veterinary Medicine (2022).
20. Elif Gokce., et al. “Positive effect of BSA supplemented soybean lecithin-based extender on liquid storage of ram semen at 50C”. Ankara Üniv Vet FakDerg 64 (2017): 313-320.
21. DL Garzon., et al. “Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of European eel sperm”. Reproduction in Domestic Animals 43 (2008): 99-105.
22. DS Penaranda., et al. “Improvement of European eel sperm cryopreservation method by preventing spermatozoa movement activation caused by cryoprotectants”. Cryobiology 59 (20095): 119-126.
23. Ma Asuncion., et al. “Optimized extenders for cryopreservation of buck semen for Artificial Insemination”. Philippine Journal of Veterinary and Animal Sciences1 (2013): 1-10.

Citation

Citation: Arjuma Khatun and Nafis I Assad. “Bovine Serum Albumin (BSA) and Foetal Bovine Serum (FBS) for Cryopreservation of Semen in Domestic Animals: Mini Review".Acta Scientific Veterinary Sciences 7.7 (2025): 14-17.

Copyright

Copyright: © 2025 Arjuma Khatun and Nafis I Assad. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.