Tran Thu Thuy¹, Luu Huynh Anh², Hua Thai Nhan³, Ly Thi Thu Lan⁴ and Nguyen Trong Ngu¹*

¹Department of Veterinary Medicine, College of Agriculture, Can Tho University, Vietnam
²Department of Animal Science, College of Agriculture, Can Tho University, Vietnam
³Department of Coastal Aquaculture, College of Aquaculture and Fisheries, Can Tho University, Vietnam
⁴School of Agriculture and Aquaculture, Tra Vinh University, Vietnam

*Corresponding Author: Nguyen Trong Ngu, Department of Veterinary Medicine, College of Agriculture, Can Tho University, Vietnam.

Received: November 11, 2021 Published: December 10, 2021

Abstract

This study was conducted to isolate and characterize bacteriophages capable of lysing Aeromonas hydrophila causing hemorrhagic septicemia in striped catfish (Pangasianodon hypophthalmus) in ponds in Dong Thap province. A total of 64 bacteriophages (58.2%) were isolated from 110 samples, with the highest value being from sludge samples (100%). When phages were tested in different pH levels (2-10), the above 64 phages were well adjusted to the pH 5-8 after 24 hours at 28^oC, representing a rate of more than 60%. In addition, in one hour, 50% of the phages could survive at different temperatures (4^oC, 20^oC, 60^oC, 80^oC, 100^oC, and 120^oC) with no effects on phages observed at 4^oC and 20^oC. From the pH and temperature tests, six phages (B.N26, B.N16, B.R18, B.B8, B.R22, and B.N1) were selected to evaluate the lysis capacity against A. hydrophila in tap and river water for 21 days in vitro. The results revealed that the bacteriophages could survive better in river water than in tap water. All six phages could be utilized to investigate the effectiveness of treating A. hydrophila-caused hemorrhagic disease in catfish in vivo.

Keywords: Aeromonas hydrophila; Bacteriophage; Isolation; pH; Striped Catfish; Temperature

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Citation

Citation: Nguyen Trong Ngu., et al. “Isolation and Characterization of Bacteriophages Against Aeromonas hydrophila Bacteria Causing Hemorrhagic Septicemia from Striped Catfish (Pangasianodon hypophthalmus)". Acta Scientific Veterinary Sciences 4.3 (2022): 56-65.

Copyright

Copyright: © 2022 Nguyen Trong Ngu., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.