Naqa Saleh Mahdi Tamimi¹*, Amir Rostami² and Hossein Esmaeili³

¹Department of Internal and preventive Medicine; College of veterinary medicine, University of Wasit. Kut, Iraq
²Department of Internal Medicine; Faculty of Veterinary Medicine; University of Tehran, Tehran, Iran
³Department of Microbiology; Faculty of Veterinary Medicine; University of Tehran, Tehran, Iran

*Corresponding Author: Naqa Saleh Mahdi Tamimi, Department of Internal and preventive Medicine; College of veterinary medicine, University of Wasit. Kut, Iraq. E-mail: naqavet2018@gmail.com, nmahdi@uowasit.edu.iq

Received: July 28, 2021; Published: August 31, 2021

Abstract

  Reptiles are shown to harbor and shed Salmonella without having any sign of illness and with notable rates that could pose a health hazard for their owners. In order to find a suitable enrichment broth for the recovery of Salmonella from these animals’ feces Rappaport Vassiliadis (RV) broth was compared with selenite F (SF). Two sets of samples were collected from 270 reptiles in Tehran province which belonged to 46 reptilian species of 22 families. The specimens included 171 cloacal swabs, 20 feces, and 79 terrarium water specimens (in which the reptile defecates) according to their availability and reptile species. Each sample was separately enriched in RV and SF enrichment broths and then inoculated in three culture media to increase the possibility of Salmonella detection (CHROMagar Salmonella, MacConkey agar, and xylosine-lysin desoxycholate) and later checked for their biochemical properties as Salmonella spp. A total of 147 (54.4%) specimens were found positive for Salmonella using both enrichment broths. The positivity rate for RV (49.6%) was significantly higher than SF (40.4%) showing that RV could be considered a better enrichment broth for Salmonella recovery in feces or cloacal swabs (p = 0.031). In addition, the reptilian feces samples were more likely to have positive culture results than cloacal swabs and terrarium water in RV and SF broths (p = 0.001 and 0.000, respectively); suggesting that, feces can be better specimens for detection of Salmonella intestinal shedding in reptiles or other animals.

Keywords: Enrichment Broth; Reptile; Salmonella

References

1. Selbitz HJ., et al. “Das Salmonellen-problem. Salmonellen als Erreger von Tierseuchen und Zoonosen”. Gustav Fischer Verlag, Jena-Stuttgart (1995): 18-51.
2. Turnbull PCB. “Food poisoning with special reference to Salmonella - its epidemiology, pathogenesis and control”. Clinical Gastroenterology 8 (1979): 586-594.
3. Woodward DL., et al. “Human salmonellosis associated with exotic pets”. Journal of Clinical Microbiology 35 (1997): 2786-2790.
4. Chiodini R J and Sundberg J P. “Salmonellosis in reptiles: a review”. American Journal of Epidemiology 113 (1981): 494.
5. Milstone AM., et al. “Alerting pregnant women to the risk of reptile-associated salmonellosis”. Obstetrics and Gynecology 107 (2006): 516-518.
6. Mermin J., et al. “Iguana and Salmonella Marina infection in children: A reflection of the increasing incidence of reptile-associated Salmonellosis in the United States”. Pediatrics 99 (1997): 399-402.
7. Cohen ML., et al. “Turtle-associated salmonellosis in the United States — effect of public health action, 1970-1976”. JAMA 243 (1980): 1247-1249.
8. Wegener HC., et al. “Pork as a source of human salmonellosis”. II International Symposium on Epidemiology and Control of Salmonella in Pork, Copenhagen (1997): 3-8.
9. Love B C and Rostagno M H. “Comparison of Five Culture Methods for Salmonella Isolation from Swine Fecal Samples of Known Infection Status”. Journal of Veterinary Diagnostic Investigation5 (2008): 620-624.
10. Michael GB., et al. “Comparison of different selective enrichment steps to isolate Salmonella sp from feces of finishing swine”. Brazilian Journal of Microbiology 34 (2003): 138-142.
11. Harvey RW and Price TH. “Salmonella isolation from reptilian faeces: a discussion of appropriate cultural techniques”. Journal of Hygiene (Lond)1 (1983): 25-32.
12. Pasmans F., et al. “Characterization of Salmonella isolates from captive lizards”. Veterinary Microbiology 110 (2005): 285-291.
13. Centers for Disease Control and Prevention (CDC). “Multistate outbreak of human Salmonella typhimurium infections associated with pet turtle exposure - United States, 2008”. Morbidity and Mortality Weekly Report 59 (2010): 191-196.
14. Rall VLM., et al. “Evaluation of three enrichment broths and five plating medium for Salmonella detection in poultry”. Brazilian Journal of Microbiology 2 (2005): 147-150.
15. Kalapothaki V., et al. “Comparison of Rappaport-Vassiliadis Enrichment Medium and Tetrathionate Brilliant Green Broth for Isolation of Salmonellae from Meat Products”. Journal of Food Protection 7 (1983): 618-621.
16. Morinˇigo MA., et al. “Laboratory study of several enrichment broths for the detection of Salmonella spp. particularly in relation to water samples”. Journal of Applied Bacteriology 74 (1993): 330-335.
17. García C., et al. “Assessment of Salmonella spp. in feces, cloacal swabs, and eggs (eggshell and content separately) from a laying hen farm”. Poultry Science7 (2011): 1581-1585.
18. Serpil KAHYA., et al. “Comparison of Different Sample Types for Salmonella Detection From Chicken Layer Breeder Flocks”. Uludağ Üniversitesi Veteriner Fakültesi Dergisi 2 (2013): 19-24.

Citation

Citation: Naqa Saleh Mahdi Tamimi., et al. “Comparison Between Two Enrichment Broths for Detection of Salmonella from Reptilian Feces and Cloacal Swabs". Acta Scientific Veterinary Sciences 3.9 (2021): 82-85.

Copyright

Copyright: © 2021 Naqa Saleh Mahdi Tamimi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.