Acta Scientific Pharmaceutical Sciences

Research Article Volume 10 Issue 1

Analytical Techniques for the Assay of Afatinib: A Review

Mukthinuthalapati Mathrusri Annapurna* and Alapati Gayathri

GITAM School of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, India

*Corresponding Author:Mukthinuthalapati Mathrusri Annapurna, GITAM School of Pharmacy, GITAM (Deemed to be University), Visakhapatnam, India.

Received: November 28, 2025; Published: December 17, 2025

Abstract

Afatinib is an antineoplastic agent used for the treatment of lung cancer. In the present study the authors have summarised the analytical methods so far published for the estimation of Afatinib in the literature.

Keywords: Afatinib; Lung Cancer

References

  1. Vavalà T. "Role of Afatinib in the treatment of advanced lung squamous cell carcinoma". Clinical Pharmacology 9 (2017): 147-157.
  2. Miller VA., et al. "Afatinib versus placebo for patients with advanced, metastatic non-small-cell lung cancer after failure of erlotinib, gefitinib, or both, and one or two lines of chemotherapy (LUX-Lung 1): a phase 2b/3 randomised trial". The Lancet Oncology5 (2012): 528-538.
  3. Gilotrif (Afatinib) (PDF). US Food and Drug Administration. (2021).
  4. Santhosh I., et al. “Method development and validation of Afatinib in bulk and pharmaceutical dosage form by UV-spectroscopic method”. Indo American Journal of Pharmaceutical Sciences3 (2018): 1569-1575.
  5. Ankita D., et al. “Development and validation of a rapid RP-HPLC method for the analysis of Afatinib in tablet dosage form using AQBD approach”. International Journal of Pharmaceutical Sciences2 (2025): 739-752.
  6. Purushottam Reddy C., et al. “Method development and validation of Afatinib using chiral HPLC”. World Journal of Pharmaceutical and Life Sciences 7 (2020): 188-197.
  7. Ravikumar V., et al. “New RP-HPLC method for the determination of Afatinib dimaleate in bulk and pharmaceutical dosage forms”. Journal of Develop Drugs4 (2015).
  8. Adnan AK., et al. “A highly efficient and sensitive LC-MS/MS method for the determination of Afatinib in human plasma: Application to a metabolic stability study”. Biomed Chromatography8 (2016): 1248-1255.
  9. Asha Deepti and Alapati Sahithi. “A sensitive bioanalytical method development and validation of Afatinib in human plasma by LC-ESI-MS/MS”. International Journal of Health SciencesS5 (2022):1376-1384.

Citation

Citation: Mukthinuthalapati Mathrusri Annapurna and Alapati Gayathri. “Analytical Techniques for the Assay of Afatinib: A Review". Acta Scientific Pharmaceutical Sciences 10.1 (2026): 15-17.

Copyright

Copyright: © 2026 Mukthinuthalapati Mathrusri Annapurna and Alapati Gayathri. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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    Authors are requested to submit manuscripts on/before February 10, 2026, for the second issue of 2026.

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