Yamini Malhotra*

Research Writing, SGSH Publications, India

*Corresponding Author:Yamini Malhotra, Research Writing, SGSH Publications, India.

Received: November 25, 2025; Published: December 15, 2025

Abstract

The present study investigates the preliminary in vitro cytotoxic and oxidative stress–inducing potential of processed amazonite extracts against the MDA MB 231 human breast cancer cell line. Cytotoxicity was evaluated using the MTT assay, while intracellular reactive oxygen species (ROS) generation was assessed qualitatively using DCFH DA fluorescence staining. Fourier Transform Infrared Spectroscopy (FTIR) was employed only for surface functional group observation and not to infer intrinsic organic composition. The results demonstrate a concentration dependent reduction in cell viability, with an IC₅₀ value of 64.85 μg/mL, indicating moderate cytotoxic activity under in vitro conditions. Qualitative ROS imaging revealed increased intracellular fluorescence in treated cells, suggesting oxidative stress involvement in cell death. However, the absence of normal cell line controls, quantitative ROS analysis, physicochemical nanoparticle characterization, and in vivo validation restricts therapeutic interpretation. This study should be considered exploratory and hypothesis generating, providing preliminary data that warrant further mechanistic, toxicological, and translational investigations before any biomedical or radiotherapeutic relevance can be proposed.

Keywords: Amazonite; MDA MB 231; Breast Cancer; MTT Assay; Reactive Oxygen Species; In vitro Cytotoxicity

References

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Citation

Citation: Yamini Malhotra. “Amazonite (A Microcline Feldspar) - An Anti-Cancerous Geo-Biotechnological Approach for MDA-MB-231: Human Breast Cancer with Validation Via ROS Anti-Oxidant Analysis". Acta Scientific Pharmaceutical Sciences 10.1 (2026): 02-14.

Copyright

Copyright: © 2026 Yamini Malhotra. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.