Ankur Kumar Singh¹, Himanshu Choudhary¹ and Ankita Raikwar²

¹B Pharm scholar, Jaipur National University, Jaipur, India
²PhD Scholar, Jaipur National University, Jaipur, India

*Corresponding Author: Ankita Raikwar, PhD Scholar, Jaipur National University, Jaipur, India.

Received: August 01, 2025; Published: August 20, 2025

Abstract

Introduction: Cassia fistula Linn., commonly known as the golden shower tree, is recognized for its rich content of bioactive phytochemicals, especially flavonoids, which are associated with a broad spectrum of pharmacological activities. This study aimed to isolate flavonoids from different parts of Cassia fistula and to evaluate the efficiency of various extraction methods in yielding high-quality flavonoid compounds. Methodology: Crude extracts of Cassia fistula were prepared using Soxhlet extraction technique. Preliminary phytochemical screening was conducted to confirm the presence of flavonoids. The extracts were subjected to isolation and purification using Thin Layer Chromatography (TLC), and further characterized by UV-Visible spectroscopy to identify specific absorption maxima indicative of flavonoid chromophores. Result: The TLC analysis revealed distinct Rf values, confirming successful separation of flavonoid constituents. UV-Visible spectroscopy confirmed the presence of flavonoids through characteristic absorption peaks. The study concludes that soxhlet is a efficient and reproducible technique for isolating flavonoids from Cassia fistula, and the findings support the potential use of such flavonoid-rich extracts in pharmacological and nutraceutical applications. Keywords: Cassia fistula; Flavonoids; Thin Layer Chromatography (TLC); UV-Visible Spectroscopy; Phytochemical Screening; Extraction

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Citation

Citation: Ankita Raikwar., et al. “A Comparative Study on the Isolation of Flavonoids from Cassia fistula and its Characterization by TLC and UV-Vis Spectroscopy". Acta Scientific Pharmaceutical Sciences 9.9 (2025): 28-35.

Copyright

Copyright: © 2025 Ankita Raikwar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.