Nazanin Zeynab Rahmani¹*, Fardin Amidi², Akbar Karimi³ and Mahdi Hadi⁴

¹Biology Department, PNU Tehran East Branch, Tehran, Iran
²Department of Anatomy, School of Medicine, Tehran University of Medicine, Tehran, Iran
³Esfahan Agriculture and Natural Resources Research and Education Center, Esfahan, Iran
⁴Department of Reproductive Biology, Pishgam Paya Zist Research and Knowledge Based Company, Tehran, Iran

*Corresponding Author: Nazanin Zeynab Rahmani, Biology Department, PNU Tehran East Branch, Tehran, Iran.

Received: March 28, 2024; Published: April 10, 2024

Abstract

Introduction: Infertility is one of the major health problems in the world. Infertility is defined as the inability to get pregnant after one year of regular intercourse, without the use of any fertility prevention methods. Infertility includes 40% men, 40% women, 10% both sexes and 10% unknown. There are various ways to treat infertility, one of them is IVF.

Materials and Methods: This study was done by using BSA and HSA serum and autologous serum from female parent mice in IVF medium. The effect of serum in the medium was performed in three groups with Zn and without Zn and three replications in each group. Data were analyzed by one-way ANOVA and then by Tukey's test using SPSS 20 software.

Results: The percentage of embryos in HSA-containing and autologous cultures was lower than the control group containing BSA (P < 0.05). The lowest percentage of embryos formed belonged to the autologous serum test group (P < 0.05). But in the medium containing zinc, no changes were found in any of the HSA, BSA and autologous serum due to the addition of zinc.

Conclusion: BSA did not show any change compared to BSA + Zn at 2Pn, BSA showed superiority over autologous and HSA.BSA + Zn showed superiority over autologous and HSA.

Keywords: IVF; Autologous Serum; Bovine Albumin Serum; Human Albumin Serum; 2PN Embryo; Zinc (Zn)

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Citation

Citation: Nazanin Zeynab Rahmani., et al. “Comparison of the Effect of Autologous Mouse Sera, Human Albumin Serum and Bovine Albumin Serum in IVF Medium, In the Presence of Zinc Supplementation on the Quality and Number of Fetuses in the 2PN Stage of Laboratory Mice".Acta Scientific Pharmaceutical Sciences 8.5 (2024): 03-08.

Copyright

Copyright: © 2024 Nazanin Zeynab Rahmani., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.