Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Research Article Volume 7 Issue 12

Effect of Cucurbita maxima Leaf Extract on Acetaminophen-induced Toxicity in Liver Function and Histoarchitecture in Adult Wistar Rats

Nwanama Emmanuel Kenechulwu1*, Ibenyenwa John Chinaza2 and Mbang Joshua Egbe3

1Department of Anatomy, Faculty of Basic Medical Sciences, Edo State University, Edo State, Nigeria
2Department of Public Health, Peace Land College of Health Sciences and Technology, Enugu, Enugu State, Nigeria
3Department of Anatomy, Faculty of Basic Medical Sciences, University of Calabar, Cross Rivers State, Nigeria

*Corresponding Author: Nwanama Emmanuel Kenechulwu, Department of Anatomy, Faculty of Basic Medical Sciences, Edo State University, Edo State, Nigeria.

Received: September 05, 2023; Published: November 07, 2023

Abstract

Context: Cucurbita maxima (Pumpkin) leaf is rich in carotenoid and flavonoid that are believed to perform protective effects. In this study, the effects of Cucurbita maxima extract are investigated in the treatment of Wistar rat liver injury induced by acetaminophen (paracetamol).

Materials and Methods: Twenty adult male Wistar rats weighing between 180 and 200 g were divided into five groups (n = 4). Normal control group; received 1ml of normal saline, negative control; paracetamol (500mg/kg) for fourteen days, experimental groups; paracetamol (500mg/kg/day) and Cucurbita maxima extract (25, 50 and 75mg/kg/day) 7 days respectively. At the end of the experiment, toxicity was measured histopathological by light microscopy and biochemically by checking levels of liver functions.

Results: The negative control group had increased level of alanine transaminase and aspartate aminotransferase and hepatocellular inflammation. When compared to the negative control, the Cucurbita maxima treatment decreased the serum levels of both aspartate aminotransferase and alanine transaminase. Additionally, hepatic tissues from our study showed that Cucurbita maxima treatment reduced liver damage caused by oxidative stress, which in turn activated autophagy.

Conclusions: Our research demonstrates that Cucurbita maxima has the potential to be a medicinal agent by protecting liver tissue from acetaminophen-induced toxicity.

Keywords: Cucurbita maxima; Hepatic-Disorder; Histoarchitecture; Paracetamol

References

  1. Cao P., et al. “Angelica sinensispolysaccharide protects against acetaminophen-induced acute liver injury and cell death by suppressing oxidative stress and hepatic apoptosis in vivo and in vitro”. International Journal of Biological Macromolecules 111(2018):1133-1139.
  2. Cai Y., et al. “Osthole prevents acetaminophen-induced liver injury in mice”. Acta Pharmacologica Sinica 1(2018):74-84.
  3. Lancaster EM., et al. “Acetaminophen hepatotoxicity: an updated review”. Archives of Toxicology 89(2015):193-199.
  4. Yan M., et al. “Mechanisms of acetaminophen-induced liver injury and its implications for therapeutic interventions”. Redox Biology 17(2018):274-283.
  5. Yang S., et al. “Geniposide protected hepatocytes from acetaminophen hepatotoxicity by down-regulating CYP 2E1 expression and inhibiting TLR 4/NF-κB signaling pathway”. International Immunopharmacology 74(2019):
  6. Wu C-T., et al. “Salvianolic acid C against acetaminophen-induced acute liver injury by attenuating inflammation, oxidative stress, and apoptosis through inhibition of the Keap1/Nrf2/HO-1 signaling”. Oxidative Medicine and Cellular Longevity (2019):
  7. Ramachandran A and Jaeschke H. “Mechanisms of acetaminophen hepatotoxicity and their translation to the human pathophysiology”. Journal of Clinical and Translational Research 3(2017): 157-169.
  8. Iorga A., et al. “Drug-induced liver injury: cascade of events leading to cell death, apoptosis or necrosis”. International Journal of Molecular Sciences5(2017):1018.
  9. Woolbright BL and Jaeschke H. “Role of the inflammasome in acetaminophen-induced liver injury and acute liver failure”. Journal of Hepatology 66(2017): 836-848.
  10. Torres S., et al. “Endoplasmic Reticulum Stress-Induced Upregulation of STARD1 Promotes Acetaminophen-Induced Acute Liver Failure”. Gastroenterology 157(2019): 552-568.
  11. Zhao, et al. “Schisandra chinensis essential oil attenuates acetaminophen-induced liver injury through alleviating oxidative stress and activating autophagy”. Pharmaceutical Biology 60.1(2022): 958-996.
  12. Gaskell H., et al. “High-Mobility Group Box-1 and Liver Disease”. Hepatology Communication 2(2018): 1005-1020.
  13. Chandrasekar R and Sivagam B. “Edible seeds medicinal value, therapeutic applications and functional properties: a review”. International Journal of Pharmacy Pharmaceutical Sciences13(2021):11-18.
  14. Musaidah M., et al. “The effect of pumpkin seeds biscuits and Moringa extract supplementation on hemoglobin, ferritin, C-reactive protein, and birth outcome for pregnant women: a systematic review”. Open Access Maced. Journal of Medical Sciences9(2021):360-365,
  15. Dotto JM and Chacha JS. “The potential of pumpkin seeds as a functional food ingredient a review”. Scientific African10(2020):1-14.
  16. Hagos M., et al. “Development of analytical methods for determination of β-carotene in pumpkin (Cucurbita maxima) flesh, peel, and seed powder samples”. International Journal of Analytical Chemistry (2022):1-11.
  17. Lestari B and Meiyanto E. “A review. The emerging nutraceutical potential of pumpkin seeds”. Indonesian Journal of Cancer Chemoprevention 9(2018):92-101.
  18. Roy S and Datta S. “A comprehensive review on the versatile pumpkin seeds (Cucurbita maxima) as a valuable natural medicine”. International Journal of Current Research7 (2015):19355-19361.
  19. Finbarrs-Bello, et al. “In vitro therapeutic potential of leaf extract of Eugenia uniflora linn on acute inflammation rat model”. Journal of Diseases and Medicinal Plants 6.2(2020):31-38.
  20. Bunchorntavakul C and Reddy KR. “Acetaminophen (APAP orN-acetyl-p-aminophenol) and acute liver failure”. Clinical Liver Disease 2(2018):325-346.
  21. McCrae JC., et al. “Long-term adverse effects of paracetamol-a review”. British Journal of Clinical Pharmacology10(2018):2218-2230.
  22. Hussain S., et al. “Cinnamon oil against acetaminophen-induced acute liver toxicity by attenuating inflammation, oxidative stress and apoptosis”. Toxicology Reports 7(2020):1296-1304.
  23. Islam MT., et al. “Effects of nerol on paracetamol-induced liver damage in Wistar albino rats”. Biomedicine and Pharmacotherapy 140(2021):
  24. Letha N., et al. “Evaluation of in vitro antioxidant activity and phytochemical screening of Croton macrostachyus Hochst. by using different solvent extracts”. American Journal PharmTech Research6(2016):73-85.
  25. Raskovic A., et al. “Hepatoprotective and antioxidant potential of Pycnogenol in acetaminophen-induced hepatotoxicity in rats”. Phytotherapy Research 33 (2018): 631-639.
  26. Koca-Caliskan U., et al. “Cuscuta arvensis beyr “Dodder”: in vivo hepatoprotective effects against acetaminophen-induced hepatotoxicity in rats”. Journal of Medicinal Food21 (2018): 625-631.

Citation

Citation: Nwanama Emmanuel Kenechulwu., et al. “Effect of Cucurbita maxima Leaf Extract on Acetaminophen-induced Toxicity in Liver Function and Histoarchitecture in Adult Wistar Rats".Acta Scientific Pharmaceutical Sciences 7.12 (2023): 25-30.

Copyright

Copyright: © 2023 et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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