Kosayev JV ¹ *, Hasanov IA² , Abushov NS ¹ , Taghi-Zade GT ¹ , Guliyev RA¹ , Ahmadov SR¹ , Zeinalov EG ¹

¹ Scientific Center of Surgery Named After Academician M.A. Topchubashov, Baku, Azerbaijan
² "Ozone" Medical Center, Ganja, Azerbaijan

*Corresponding Author: Kosayev JV, Scientific Center of Surgery Named After Academician M.A. Topchubashov, Baku, Azerbaijan.

Received: December 18, 2025; Published: February 24, 2026

Objective: To immunocytochemically assess the CD25 (fixed cell receptor interleukin-2) positivity and TNF-α positivity of peripheral blood cells in patients with critical ischemia of the lower extremities (CILE) under intravenous laser irradiation of blood (ILIB) for indirect revascularization.

Material and Methods: The study was carried out in 66 patients with CILE who underwent indirect limb revascularization due to the impossibility of reconstructive operations and endovascular revascularization. In the perioperative period, 34 patients (the control group) underwent conventional treatment without ILIB. In 32 patients (the main group), ILIB was performed with the Mustang 2000 and Mustang 2000+ devices. CILE developed against the background of thromboangiitis obliterans and atherosclerosis obliterans.

Results: Prior to treatment, patients had a significantly increased number of CD25+ monocytes, granular leukocytes, and monocytes (p < 0.05). In the control group, by the end of treatment, the number of CD25 + granular leukocytes (p < 0.05) decreased, but the increased expression of CD25 by monocytes and lymphocytes did not normalize. The frequency of abnormal CD25-positive thrombo-leukocyte agregates remains stable. In patients of the main group, the expression of CD25 remains elevated in lymphocytes and monocytes, but is completely normalized in granular leukocytes (p < 0.001), and thrombo-leukocyte agregates are reduced to a minimum. Increased number of TNF-α – positive formed elements (monocytes, granulocytes, lymphocytes) and TNF-α – positive thrombo-leukocyte agregates (p < 0.05). After treatment, the number of TNF-α-positive cells in the study group decreased significantly compared to the control group.

Conclusion: The use of ILIB in the perioperative period with indirect revascularization in patients with CILE completely normalizes CD25 expression in granular leukocytes, reduces the number of TNF-α-positive formed elements, the number of CD25 positive and TNF-α-positive thrombo-leukocyte agregates in peripheral blood.

Keywords: Lower Extremity Critical Ischemia; Indirect Revascularization; Intravenous Laser Irradiation of Blood; CD25 Positivity of Peripheral Blood Cells; TNF-α Positivity of Peripheral Blood Cells

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Citation

Citation: Kosayev JV., et al. “CD25 and TNF-α in Patients with Critical! Ischemia of the Lower Extremities Under Conditions of Intravenous Laser Irradiation of Blood with Indirect Revascularization". Acta Scientific Medical Sciences 10.3 (2026): 30-33.

Copyright

Copyright: © 2026 Kosayev JV., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.