Basil S Alnofaie^1,2*, Mohamed H Zainy¹ and Noor M Bataweel³

¹Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia
²Department of Biology, College of Science and Art at Khulis, University of Jeddah, Saudi Arabia
³King Fahd Medical Research Center, King Abdul-Aziz University, Jeddah, Saudi Arabia

*Corresponding Author: Basil S Alnofaie, Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

Received: May 23, 2024; Published: June 13, 2024

Abstract

Jeddah is the second largest city in Saudi Arabia and is located on the Red Sea coast (western seaboard). It has the largest seaport along the Red Sea coast. Rapid industrialization and urbanization in the Jeddah area have raised concerns over potential impacts on public health and the environment. Fish alone accounts for over 80% of the animal protein in our diet. But occasionally, infections or intoxication from eating fish might lead to disease. The general pollution of the aquatic environment is thought to be reflected in it. Fish are conditioned by their surroundings, therefore it stands to reason that if the habitat in which they grow and are harvested is chemically or microbiologically polluted, the fish are likewise polluted. These fish can spread disease to nearby persons through handling while being transported to the landing center and wholesale market. Monitoring the microbial contaminants in commonly consumed fish will thus help safeguard the public from any potential adverse risks. This study isolated several species of bacteria from specific kinds of fish raw Greasy grouper fish ( Hamoor collected from the Jeddah central fish market and identified them molecularly. Fish samples were collected individually in a clean polyethylene bag and transferred immediately to the laboratory in refrigerated condition. Then fish samples were dissected, and bacteria were isolated on three media. McConkey agar, TCBS agar, and Blood agar. Extracted DNA of samples isolated 16S rRNA gene sequences of all the (41) bacteria isolated were amplified.

 Keywords: Raw Grouper Fish; Media; 16srRNA Gene; PCR; Susceptibility

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Citation

Citation: Basil S Alnofaie., et al. “Microbial and Molecular Studies of Microbes in Raw Grouper Fish (Hamoor) in Jeddah Province”.Acta Scientific Medical Sciences 8.7 (2024): 104-113.

Copyright

Copyright: © 2024 Basil S Alnofaie., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.