Department of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, Turkey
*Corresponding Author: Dilek Tekdal, Department of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, Turkey.
Received: September 22, 2021; Published: October 15, 2021
Tissue culture techniques are needed in many areas of plant studies. In this study, it is aimed to reveal the potential of a vegetable such as lentils, which is an essential plant in human nutrition, to be multiplied by the ovule-embryo culture technique, which is one of the tissue cultures methods. Ovule-embryo culture method minimizes embryo deterioration during the early stages of development, reduces breeding time, and allows for the effective multiplication of numerous plants from a single embryo. Lentil plant (Lens culinaris Medik.) ovule explants were used as plant material. Embryos extracted from ovular integuments and cultured on Murashige and Skoog’s medium supplemented with various concentrations and combination of Kinetin and 2,4-D. As a result of the study, the best callus formation rate (60%) was obtained from MS nutrient medium containing Kinetin (1 mg mL-1) and 2,4-D (0.5 mg mL-1).
Keywords: Mersin; Ovule; Propagation; Lens culinaris
Citation: Dilek Tekdal. “Ovule-Embryo Culture in Lentil (Lens culinaris Medikus)". Acta Scientific Agriculture 5.11 (2021): 09-11.
Copyright: © 2021 Dilek Tekdal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.