Blandine Pulcherie Tamatcho Kweyang^1,2, Antoine Tamsa Arfao^1,3*, Daniel Ébang Menye⁴, Anayelle Yemdji Tanekem^1,2, Mohammadou Sanoussi^1,2, Ange Leslie Koagne Nembot Nelong¹, Jocelyne Madaye³, Issah Nji Mongou Moifon¹, Alexandra Geordie Ngaldeu Noupoue⁵, Marie Nickelle Ngoupemgbie Njiawouo^1,2 and Samira Ngayen Ngoupayou^1,2

¹Hydrobiology and Environment Laboratory, University of Yaoundé 1, Faculty of Sciences, P.O. Box 812 Yaounde, Cameroon
²Department of Microbiology, University of Yaoundé 1, Faculty of Sciences, P.O. Box 812 Yaounde, Cameroon
³Zoology Laboratory, Faculty of Sciences, University of Maroua, and Medical Analysis and Application Laboratory, Higher Institute of Health Science, Technician and Management of Garoua (ISSTSM), Garoua, Cameroon
⁴Département des Sciences de la Vie et de la Terre, École Normale Supérieure, Université de Maroua, B.P. 55, Maroua, Cameroun
⁵Department of Aquatic Ecosystems Management, Institute of Fisheries and Aquatic Sciences, University of Douala, Douala, Cameroun

*Corresponding Author: Antoine Tamsa Arfao, Hydrobiology and Environment Laboratory, University of Yaounde 1, Faculty of Sciences, P.O. Box 8318 Yaounde, Cameroon.

Received: September 30, 2025; Published: June 30, 2026

Abstract

Fish is an important source of animal protein for people in sub-Saharan Africa. These fish can be contaminated by bacteria called Vibrio, which can make people very sick. The main aim of this study was to look at the different types of Vibrio bacteria found in fish products (such as sea bass, carp and mackerel) sold in three markets in Yaoundé (Mfoundi, Acacia and Mendong). Isolations were performed on TCBS medium, followed by identification using macroscopic, microscopic and biochemical methods. We checked how well the antibiotics would work using the agar diffusion method (antibiogram) in accordance with CASFM recommendations (2023). Seven antibiotics were chosen for the analysis: amoxicillin, cefotaxime, chloramphenicol, amoxicillin + clavulanic acid, ciprofloxacin, nalidixic acid and doxycycline. In total, 61 types of bacteria were identified, which came from five different groups. Vibrio alginolyticus (34.43%), V. parahaemolyticus (32.79%), V. cholerae (19.67%), V. fluvialis (8.20%) and V. mimicus (4.92%). The highest Vibrio levels were found in the mackerel skins sample, with an average of 5.28 log10 (UFC/g), while the lowest was found in the mackerel inner organs sample, with an average of 0 log10 (CFU/g). Tests showed that the bacteria were resistant to seven different antibiotics (AMC, AMX, CTX, CHL, DOX, CIP, NAL). There were also some cases of the bacteria being resistant to more than one antibiotic. V. cholerae was resistant to AMC and AMX in several markets. These results show that better health checks are needed on fishery products and that we need to watch how resistant antimicrobials are in Cameroonian markets.

Keywords: Vibrio Species; Diversity; Fisheries Products; Antibiotic Susceptibility

References

1. “L’Etat de la sécurité alimentaire et de la nutrition dans le monde”. Organisation des nations unies pour l’alimentation et l’agriculture, fonds international de développement agricole (2022): 40.
2. “Situation de la production et des importations du sous-secteur élevage pêches et industries animales”. (2024): 28.
3. “La situation mondiale de l’alimentation et de l’agriculture”. Collection 37 (2006): 1-207.
4. Djama T and Abo’o NP. “La gestion des pêches et de la biodiversité”. Support de cours ACP-UE, Dakar, Sénégal, du 12 - 23 Avril 1999 : aperçu de la pêche Camerounaise. (1999).
5. “Optimiser la production des poissons par la construction des étangs, la production des alevins et la gestion des étangs suivant des techniques appropriées” (Programme national de Vulgarisation et de Recherche Agricoles) (2003): 23.
6. Forsythe SJ. “Food safety Assurance in the European Union”. Food safety resources on the world wide web, Codex- Food Hygiene (2000): 23.
7. Tamatcho KBP., et al. “Characterization and Antimicrobial Susceptibility Patterns of Vibrio cholerae and Vibrio cholerae O1 Isolated in Tropical Waters: A Contribution to Cholera Disease Prevention in Equatorial Region”. Science and Education Publishing 9 (2021): 599-606.
8. Cohen N and karib H. “Vibrio spp. dans les produits de la pêche: Risques et prévention”. Les Technologies De Laboratoire 3 (2007): 4-10.
9. “Risk assessment tools for Vibrio parahaemolyticus and Vibrio vulnificus associated with seafood”. (Microbiolgical Risk assessment Series N°.20). World Health Organization. Iris who int. (2020).
10. “Multi-country outbreak of cholera, external situation report”. World Health Organization. Iris who int. (2024).
11. CCOUSP (Centre de Coordination des Opérations d’urgence de Santé Publique). “Situation cholera Cameroun” (2024).
12. Guévart E., et al. “Evolution de la sensibilité de Vibrio cholerae O1 après utilisation prolongée d’antibiotiques en traitement et en prophylaxie au cours de l’épidémie de choléra de Douala (Cameroun) 2004”. Médecine et Maladies Infectieuses 36 (2006): 329-334.
13. Holt J.G., et al. “Berger.’sBriger manual of determinative bacteriology”. Lippincott Williams and Wilkins, 9^th Philadelphia Baltimore New York London Buenos Aires Hong kong Sydney (2000): 24.
14. CASFM (Comite de l’Antibiogramme de la Societe Francaise de Microbiologie). CA-SFM/EUCAST 2023, Societe Francaise de Microbiologie 177 (2023).
15. NCCLS (National Committee for Clinical Laboratory Standards). “Performance Standard for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals”. Approved Standard, Second Edition, M31-A2. (2002).
16. Fournier JM and Quilici ML. “Infections à Vibrions non cholériques”. Encyclopédie. Médico. Chirurgicale. (Éditions), Maladies infectieuses. 8 (2002): 1-7.
17. Parveen S., et al. “Seasonal distribution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and waters”. International Journal of Food Microbiology 128 (2008): 354-361.
18. Toule AC., et al. “Prévalence et diversité des souches de Vibrio dans les eaux et les poissons des fermes aquacoles de la lagune Ebrié”. International Journal of Progressive Sciences and Technologies (IJPSAT) 46 (2024): 307-316.
19. Eddabra R. “Evaluation de la contamination bactériologique des eaux usées des stations d’épuration du grand Agadir : isolement, caractérisation moléculaire et antibiorésistance des espèces du genre Vibrio”. (Thèse de doctorat). Université IBN Zohr, Faculté des Sciences d’Agadir et Université de Strasbourg, Ecole Doctorale Sciences de la Vie et de la Santé (2011).
20. Iwanaga M., et al. “Antibiotic resistance conferred by a class I integron and SXT constin in Vibrio cholerae O1 strains isolated in Laos”. Antimicrobe, Agents Chemotherapy 48 (2004): 2364-2369.
21. Ahmed AM., et al. “A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis”. FEMS Microbiology Letter 242 (2005): 241-247.

Citation

Citation: Antoine Tamsa Arfao., et al. “Profile and Antibiotic Susceptibility of Vibrio Bacteria Associated with Some Fishery Products Sold in Certain Mar- kets of Yaoundé, Cameroon (Central Africa)". Acta Scientific Microbiology 9.7 (2026): 11-19.

Copyright

Copyright: © 2026 Antoine Tamsa Arfao., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.