Francky Love Avoulou^1,4,5, Marie Grace Clarisse Edzimbi^1,3, Esther Del Florence Moni Ndedi^1,3, Blandine Pulchérie Tamatcho Kweyang¹*, Aristide Nguele Toussaint⁴, Augustine Asakizi Nji⁵, Jean Paul Assam Assam^1,3* and Veronique Penlap Beng^2,3

¹Department of Microbiology, Faculty of Sciences, University of Yaoundé 1, BP 812, Cameroon
²Department of Biochemistry, Faculty of Sciences, University of Yaoundé 1, Cameroon
³Tuberculosis and Pharmacology Research Laboratory, Nkolbisson Biotechnology Centre, Yaoundé, Cameroon
⁴Department of Biomedical Science, Adventist University Cosendai, Nanga-Eboko, Cameroon
⁵Kesmonds International University, Cameroon

*Corresponding Author: Blandine Pulchérie Tamatcho Kweyang, Department of Microbiology, Faculty of Sciences, University of Yaoundé 1, BP 812, Cameroon and Jean Paul Assam Assam, Department of Microbiology, Faculty of Sciences, University of Yaoundé 1, BP 812, Cameroon.

Received: October 21, 2025; Published: November 07, 2025

Abstract

The aim of this study was to evaluate the antimycobacterial and antibiofilm potential of the essential oils of the bark Duguetia confinis [(Engl & Diels) Chatrou] and the rhizomes Vetiveria zizanioides (L). The essential oils were extracted by hydrodistillation and tested on the strains of mycobacteria, namely Mycobacterium tuberculosis H37Rv with ATCC (American Type Cells Cultures) code 2794 and a clinical isolate. The technique used for antimycobacterial tests is that of microdilution in liquid media for the determination of MIC and CMB inhibition parameters on planktonic cells. Then, the biofilm was induced by liquid culture on a polystyrene support. Finally, the biofilm inhibition parameters were determined in liquid media (CMIB and CMEB) and a comparison of the activity of planktonic cell inhibition parameters and biofilm inhibition parameters was made. The essential oils were obtained with a yield of 0.03% for Duguetia confinis and 0.26% for Vetiveria zizanioides. The minimum inhibitory concentrations (MIC) were 156.25 μg/mL for V. zizanioides on both the reference strain and clinical isolate, and 1250 μg/mL for D. confinis on both, showing bactericidal activity. The activity of the recorded biofilm inhibition parameters shows that Both D. confinis and V. zizanioides showed notable biofilm inhibition, with D. confinis at 156.25 μg/mL on the clinical isolate with a MIBC of 156.25 μg/mL on the clinical isolate. The activity of these EOs confirms the use of these plants for the treatment of respiratory tract diseases in traditional medicine and the results of the activity of D. confinis provide data missing in the literature review. These results show the potential antimycobacterial of the EOs tested and what could be a promising alternative solution for the treatment of tuberculosis.

Keywords: Vetiveria zizanioides; Duguetia confinis; Essential Oils; Antimycobacterial Activity; Anti Biofilm Activity

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Citation

Citation: Blandine Pulchérie Tamatcho Kweyang., et al. “Study of the Antimycobacterial and Anti-Biofilm Activity of the Essential Oils of Duguetia confinis [Engl & Diels (Chatrou)] and of Vetiveria zizanioides (L.)".Acta Scientific Microbiology 8.12 (2025): 39-47.

Copyright

Copyright: © 2025 Blandine Pulchérie Tamatcho Kweyang., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.