Acta Scientific Microbiology

Research Article Volume 8 Issue 10

Decolourization of Malachite Green Dye by Bacteria Isolated from Drainage Effluent

Sheetal Sahare1,2, Ram Prasad Kushvaha1, Renu Mishra2, Urmila Meghwal1 and Sunil Kumar Snehi1*

1Molecular Plant Virology Lab, Department of Microbiology, Barkatullah University, Bhopal (Madhya Pradesh), India
2Department of Botany and Microbiology, Sri Sathya Sai College for Women, Bhopal (Madhya Pradesh), India

*Corresponding Author: Dr. Sunil Kumar Snehi, Molecular Plant Virology Lab, Department of Microbiology, Barkatullah University, Bhopal (Madhya Pradesh), India.

Received: September 15, 2025; Published: September 23, 2025

Abstract

The widespread use of synthetic dyes, particularly malachite green (MG), in various industries has led to significant environmental pollution, necessitating effective and eco-friendly remediation strategies. This study focused on the isolation and identification of bacterial strains capable of degrading malachite green dye. A total of seven bacterial isolates were obtained from a drainage canal of New Market, Bhopal (Madhya Pradesh). Preliminary screening on dye-containing media identified two potent isolates Pseudomonas otitidis and Aeromonas hydrophila, exhibiting significant degradation capabilities. Biodecolorization Assay confirmed that isolate Pseudomonas otitidis achieved the highest decolorization efficiency of 80% in optimized condition (72 hours, 35°C), while Aeromonas hydrophila reached 76% under similar conditions for the malachite green dye. BLASTn analysis confirmed ≥ 99.86 and 100 percentage sequence similarity with known bacterial strains. Overall, the findings highlight Pseudomonas otitidis and Aeromonas hydrophila as promising bacteria for the bioremediation of malachite green contaminated environments, with Pseudomonas otitidis showing exceptional efficiency and potential for application in sustainable wastewater management strategies

Keywords: Malachite Green; Decolorization; Pseudomonas otitidis; Aeromonas hydrophila; Wastewater Management

References

  1. Jadhav Indrani., et al. "Microorganism-based treatment of azo dyes”. Journal of Environmental Science and Technology2 (2016): 188.
  2. Roy Dipankar Chandra., et al. "Bioremediation of malachite green dye by two bacterial strains isolated from textile effluents”. Current Research in Microbial Sciences1 (2020): 37-43.
  3. Berradi Mohamed., et al. "Textile finishing dyes and their impact on aquatic environs”. Heliyon11 (2019).
  4. El-Bendary Magda A., et al. "Efficient malachite green biodegradation by Pseudomonas plecoglossicide MG2: process optimization, application in bioreactors, and degradation pathway”. Microbial Cell Factories1 (2023): 192.
  5. Pandey Deepshikha., et al. "Bioremoval of toxic malachite green from water through simultaneous decolorization and degradation using laccase immobilized biochar”. Chemosphere297 (2022): 134126.
  6. de Almada Vilhena Andryo O., et al. "The synthetic dye malachite green found in food induces cytotoxicity and genotoxicity in four different mammalian cell lines from distinct tissues w”. Toxicology Research4 (2023): 693-701.
  7. Kushvaha Ram Prasad., et al. "Biodegradation of Synthetic Dyes by Bacteria Isolated from Textile Industry Water Effluent”. Methodology (2019).
  8. Ahmed Hussein M., et al. "Effective chemical coagulation treatment process for cationic and anionic dyes degradation”. Egyptian Journal of Chemistry8 (2022): 299-307.
  9. Fawzy Mariam E., et al. "Chicken bone ash as a cost-effective and efficient adsorbent for phenol removal from aqueous solution”. Desalination and Water Treatment281 (2023): 255-264.
  10. Parshetti Ganesh., et al. "Biodegradation of Malachite Green by Kocuria rosea MTCC 1532”. Acta Chimica Slovenica4 (2006).
  11. Du Lin-Na., et al. "Biodegradation of malachite green by Pseudomonas sp. strain DY1 under aerobic condition: characteristics, degradation products, enzyme analysis and phytotoxicity”. Ecotoxicology2 (2011): 438-446.
  12. Pourbabaee AA., et al. "Biodegradation of malachite green by Klebsiella Terrigenaptcc 1650: the critical parameters were optimized using Taguchi optimization method”. Journal of Bioremediation and Biodegradation Open Access 4 (2013): 175.
  13. Vijayalakshmidevi S R and Karuppan Muthukumar. "Biodegradation of malachite green by Ochrobactrum sp”. World Journal of Microbiology and Biotechnology2 (2014): 429-437.
  14. Tao Yanbin., et al. "Biological decolorization and degradation of malachite green by Pseudomonas sp. YB2: process optimization and biodegradation pathway”. Current Microbiology10 (2017): 1210-1215.
  15. Kabeer Farha Arakkaveettil., et al. "Biodegradation of malachite green by a newly isolated Bacillus vietnamensis sp. MSB17 from continental slope of the Eastern Arabian Sea: enzyme analysis, degradation pathway and toxicity studies”. Bioremediation Journal4 (2019): 334-342.
  16. Song Jinlong., et al. "Pathway and kinetics of malachite green biodegradation by Pseudomonas veronii”. Scientific Reports1 (2020): 4502.
  17. Alaya Vasudhaudupa., et al. "Decolorization of Malachite green dye by Stenotrophomonas maltophilia a compost bacterium”. Bulletin of the National Research Centre1 (2021): 81.
  18. Engelkirk Paul G and Janet L Duben-Engelkirk. “Laboratory diagnosis of infectious diseases: essentials of diagnostic microbiology”. Lippincott Williams and Wilkins, (2008).
  19. Tamura Koichiro., et al. "MEGA11: molecular evolutionary genetics analysis version 11”. Molecular Biology and Evolution7 (2021): 3022-3027.
  20. Bakar Norshila Abu., et al. "Physico-chemical water quality parameters analysis on textile”. IOP conference series: earth and environmental science. 498.1 (2020).
  21. Khan Razia., et al. "Microbial decolorization and degradation of synthetic dyes: a review”. Reviews in Environmental Science and Bio/Technology1 (2013): 75-97.
  22. Lade Harshad., et al. "Low‐cost biodegradation and detoxification of textile azo dye CI Reactive Blue 172 by Providencia rettgeri strain HSL1”. Journal of Chemistry1 (2015): 894109.
  23. Tony Bella Devassy., et al. "Decolorization of textile azo dyes by aerobic bacterial consortium”. International Biodeterioration and Biodegradation4 (2009): 462-469.
  24. Joshi Pradnya A and Kirti J Mhatre. "Microbial efficiency to degrade Carbol fuchsin and Malachite green dyes”. Advances in Applied Science Research 6 (2015): 85-88.
  25. Wanyonyi WC., et al. "Biodegradation and detoxification of malachite green dye using novel enzymes from bacillus cereus strain KM201428: kinetic and metabolite analysis. Energy Procedia 119 (2017): 38-51.

Citation

Citation: Sunil Kumar Snehi.,et al. “Decolourization of Malachite Green Dye by Bacteria Isolated from Drainage Effluent".Acta Scientific Microbiology 8.10 (2025): 19-28.

Copyright

Copyright: © 2025 Sunil Kumar Snehi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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