SR Kankonkar*, SM Bandekar, Suresh Mandrekar, Milind Karapurkar, Dhawal Faterpenkar, Kiran Gawas and Divya S Francisco Couto

Department of Histocompatibility and Tx Immunology-Pathology Dept, Goa Medical College and Hospital, Bambolim, Goa, India

*Corresponding Author: SR Kankonkar, Department of Histocompatibility and Tx Immunology-Pathology Dept, Goa Medical College and Hospital, Bambolim, Goa, India.

Received: August 22, 2025; Published: September 22, 2025

Abstract

Ankylosing Spondylitis (AS) is a disease which primarily affects spine along with other joints. The vertebrae in some cases fuse together and form a rigid structure. 500 clinically symptomatic suspected cases of AS and a few Auto-immune disorders/diseases such as, Psoriasis, Rheumatoid arthritis, Reiter's syndrome, Uveitis, diseases etc. were referred for detection and association of HLA-B27 antigens/alleles/genes/epitopes, including HLA-B7, B40, B 22, B 13, genes. 70 normal subjects, were also studied.

The present study, was carried out using Flow Cytometer (FCM) and PCR-molecular (sequence specific oligonucleotide) techniques. Our findings show a considerable and consistent association of HLA-B27 antigen with AS, irrespective of the community and hygiene or socio-economic conditions.

We found, the people in the age group of 14-40 years were most vulnerable, when severity of disease taken into consideration. Males members showed preponderance over females in positivity with HLA-B27.

Therefore, detection of HLA-B27 could help in diagnosis of Ankylosing Spondylitis.

AS patients and those suffering from other auto-immune diseases such as rheumatoid arthritis, psoriasis, Reiter’s syndrome and uveitis. A few cases with inflammatory bowel diseases, colitis, eczema, bacillary or fungal infection, were also found to be positive.

Keywords: Histocompatibility Complex (MHC); Human Leukocyte Antigens (HLA)

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Citation

Citation: SR Kankonkar., et al. “Association of HLA-B*27, Sub Types with Ankylosing Spondylitis and Other Auto-Immune Diseases Using Flow Cytometry and DNA PCR Molecular Typing".Acta Scientific Microbiology 8.10 (2025): 07-18.

Copyright

Copyright: © 2025 SR Kankonkar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.