Mahfuja Majid¹*, Farzana Pervin² , Sayontoni Projna³ and Badrul Islam⁴*

¹Institute of Biological Sciences, University of Rajshahi, Bangladesh
²Department of Biochemistry and Molecular Biology, University of Rajshahi, Bangladesh
³Research Associate, Department of Dental Public Health, City Dental College and Hospital, Dhaka, Bangladesh
⁴Natural Products Research Division, BCSIR, Rajshahi Laboratories, Bangladesh Council of Scientific and Industrial Research, Bangladesh

*Corresponding Author: Badrul Islam, Natural Products Research Division, BCSIR, Rajshahi Laboratories, Bangladesh Council of Scientific and Industrial Research, Bangladesh.

Received: August 18, 2025; Published: August 27, 2025

Abstract

Background: Abroma augusta is used extensively in traditional medicine, but little is known about its antimicrobial components.

Objective: The objective is to isolate, characterize and assess antimicrobial components from chloroform leaf extracts of A. Agusta.

Methods: The leaves were extracted using chloroform and then fractionated using column chromatography based on activity. IR, ¹H-NMR, and ¹³C-NMR spectroscopy were used to provide structural information about the isolated molecules. Antimicrobial activity was evaluated using disc diffusion and MIC tests against pathogenic fungus and both Gram-positive and Gram-negative bacteria.

Results: Octacosanol and lupeol were the two chemicals that were purified. MIC values ranging from 32 to 128 μg/ml, depending on the test organisms, indicated that both exhibited antibacterial activity. Antifungal activity was also observed against Candida albicans, Aspergillus niger, and Fusarium vasinfectum.

Conclusion: A. augusta's ethnomedicinal use and promise as a source of natural antimicrobial agents are supported by the antimicrobial qualities of octacosanol and lupeol.

Keywords: Abroma augusta; Octacosanol; Lupeol; Antimicrobial Activity; Natural Products

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Citation

Citation: Mahfuja Majid., et al. “Characterization of Antimicrobial Compounds from Abroma augusta Leaf Extract".Acta Scientific Microbiology 8.9 (2025): 39-57.

Copyright

Copyright: © 2025 Mahfuja Majid., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.