Osunla Ayodeji Charles*

Department of Microbiology, Adekunle Ajasin University Akungba Akoko, Nigeria

*Corresponding Author: Osunla Ayodeji Charles, Department of Microbiology, Adekunle Ajasin University Akungba Akoko, Nigeria.

Received: June 18, 2025; Published: July 28, 2025

Abstract

The provision of safe drinking water remains a critical global challenge, particularly in rural communities that depend on groundwater sources. This study conducted a snapshot survey to determine the occurrence and antibiotic resistance profiles of Staphylococcus species in selected wells from Akungba-Akoko, Nigeria. Twelve well water samples were examined using standard microbiological methods, with antibiotic susceptibility testing performed using disc diffusion methods against six commercially available antibiotics: penicillin G, trimethoprim/sulphonamide, vancomycin, tetracycline, streptomycin, and chloramphenicol. Results showed that seven (58.33%) of the well water samples were contaminated with Staphylococcus spp., with 71% of isolates being coagulase-positive. Antibiotic resistance analysis revealed that 23.5% of isolates were resistant to penicillin G, 11.7% to tetracycline, and 5.9% each to vancomycin and streptomycin. No resistance was observed against trimethoprim/sulphonamide and chloramphenicol. These findings indicate that wells used as drinking water sources in Akungba-Akoko harbor antibiotic-resistant Staphylococcus strains, posing potential public health risks through possible horizontal gene transfer to gastrointestinal tract bacteria in consumers.

Keywords: Staphylococcus Species; Well Water Contamination; Antibiotic Resistance; Groundwater Quality; Public Health

References

1. Abulreesh HH and Organji SR. “Water quality assessment and occurrence of antibiotic-resistant bacteria in drinking water sources”. Environmental Science and Pollution Research8 (2011): 1319-1326.
2. Adesoji AT., et al. “Prevalence of antibiotic-resistant Staphylococcus aureus in well water samples from Dutsin-Ma community, Katsina State, Nigeria”. African Journal of Microbiology Research15 (2019): 265-272.
3. Akinola SA., et al. “Antibiotic resistance patterns of Staphylococcus aureus isolated from automated teller machines in Ilorin, Nigeria”. Journal of Medical Microbiology4 (2022): 001527.
4. Aklilu E., et al. “Methicillin-resistant Staphylococcus aureus (MRSA) in animal products and the environment: A review”. Asian Pacific Journal of Tropical Biomedicine7 (2013): 562-569.
5. Basset P., et al. “Very low prevalence of meticillin-resistant Staphylococcus aureus carrying the mecC gene in western Switzerland”. Journal of Hospital Infection4 (2011): 319-320.
6. Bayode MT., et al. “Multiple antibiotic resistant (MAR) index and detection of qnrS and qnrB genes in bacterial consortium of urine samples from clinical settings”. European Journal of Biological Research1 (2021): 45-56.
7. Bello OO. “Assessment of groundwater quality in rural communities of Southwestern Nigeria”. African Journal of Environmental Science and Technology8 (2013): 816-821.
8. Bitrus AA., et al. “Antimicrobial resistance and genotypic diversity of Staphylococcus aureus isolated from cattle, chicken and goats in Malaysia”. Tropical Animal Health and Production5 (2017): 1087-1097.
9. Bitrus AA., et al. “Methicillin-resistant Staphylococcus aureus in food-producing animals and products: A review”. Food Control 84 (2018): 205-214.
10. Bosch T., et al. “Changes in the molecular epidemiology of methicillin-resistant Staphylococcus aureus bloodstream infections in a Dutch university medical center”. PLoS One7 (2015): e0132136.
11. “Staphylococcal food poisoning from canned mushrooms—New York”. Morbidity and Mortality Weekly Report 21.32 (1972): 275-276.
12. Chakraborty R and Nishith KP. “Laboratory manual of microbiology”. New Central Book Agency (2014).
13. Chokpa M P. “Assessment of groundwater quality in rural areas of Benue State, Nigeria”. International Journal of Water Resources and Environmental Engineering4 (2018): 45-52.
14. Chuang YY and Huang YC. “Molecular epidemiology of community-associated meticillin-resistant Staphylococcus aureus in Asia”. The Lancet Infectious Diseases8 (2013): 698-708.
15. Clinical and Laboratory Standards Institute. “Performance standards for antimicrobial susceptibility testing (29^th)”. CLSI document M100. Clinical and Laboratory Standards Institute (2019).
16. Courvalin P. “Vancomycin resistance in gram-positive cocci”. Clinical Infectious Diseases 42 (2006): S25-S34.
17. Cox G and Wright GD. “Intrinsic antibiotic resistance: mechanisms, origins, challenges and solutions”. International Journal of Medical Microbiology6-7 (2013): 287-292.
18. den Heijer CD., et al. “Prevalence and resistance of commensal Staphylococcus aureus, including meticillin-resistant S aureus, in nine European countries: a cross-sectional study”. The Lancet Infectious Diseases5 (2013): 409-415.
19. Department of Health London. “UK five year antimicrobial resistance strategy 2013 to 2018”. Department of Health and Social Care (2013).
20. “Scientific opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals”. EFSA Journal 9.8 (2011): 2322.
21. Ephraim KD., et al. “Microbiological quality assessment of borehole water in selected rural communities in Delta State, Nigeria”. International Journal of Current Microbiology and Applied Sciences2 (2021): 123-135.
22. Faria C., et al. “Antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water”. Science of the Total Environment12 (2009): 3876-3882.
23. Fawole MO and Oso BA. “Laboratory manual of microbiology”. (4^th) Spectrum Books Limited (2007).
24. Foster T J., et al. “Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus”. Nature Reviews Microbiology1 (2014): 49-62.
25. Foster AJ., et al. “Role of fibronectin-binding proteins in Staphylococcus aureus pathogenesis”. Microbiology Spectrum4 (2017): 10-1128.
26. Getso B U., et al. “Assessment of groundwater quality in Kano metropolis, Nigeria”. Environmental Earth Sciences3 (2018): 1-15.
27. Govindarajan M and Senthilnathan J. “Assessment of physico-chemical quality of groundwater in Thirumanimuthar sub-basin, Tamil Nadu, India”. Applied Water Science3 (2014): 253-259.
28. Jacquemyn Y., et al. “Multidrug resistance in Staphylococcus aureus: A global challenge. International Journal of Antimicrobial Agents3 (2013): 187-194.
29. Lamy B., et al. “How well do routine antimicrobial susceptibility test methods correlate with minimum inhibitory concentration methods for non-fastidious bacteria?” Journal of Antimicrobial Chemotherapy11 (2012): 2638-2645.
30. Luzzago C., et al. “Clonal diversity, virulence factor encoding genes and antimicrobial resistance in clinical Staphylococcus aureus isolates from bovine mastitis in Lombardy (Northern Italy)”. Veterinary Microbiology3-4 (2014): 382-385.
31. McAleese F., et al. “Overexpression of genes of the cell wall stimulon in clinical isolates of Staphylococcus aureus exhibiting vancomycin-intermediate- aureus-type resistance to vancomycin”. Journal of Bacteriology 188.3 (2006): 1120-1133.
32. Moyo N A. “An analysis of the chemical and microbiological quality of ground water from boreholes and shallow wells in Zimbabwe”. Physics and Chemistry of the Earth 66 (2013): 27-32.
33. Nguyen F., et al. “Tetracycline antibiotics and resistance mechanisms”. Biological Chemistry5 (2014): 559-575.
34. Noto M J. “Mechanisms of β-lactam antimicrobial resistance and epidemiology of multidrug-resistant Enterobacteriaceae in Central New York State”. Dissertation Abstracts International5 (2008): 2563.
35. Nowrouzian F L., et al. “Adhesin and superantigen genes and the capacity of Staphylococcus aureus to colonize the infantile gut”. Journal of Infectious Diseases5 (2013): 714-724.
36. Obioma A., et al. “Assessment of seasonal variation of groundwater quality around active dumpsite in Chanchaga, Niger State using multivariate statistical approach”. Applied Water Science1 (2017): 191-205.
37. Odeyemi AT., et al. “Bacteriological quality of water sources in rural communities of Osun State, Nigeria”. Global Advanced Research Journal of Environmental Science and Toxicology1 (2012): 1-5.
38. Okonko IO., et al. “Microbiological and physicochemical analysis of different water samples used for domestic purposes in Abeokuta and Ojota, Lagos State, Nigeria”. African Journal of Biotechnology5 (2008): 617-621.
39. Osarenmwinda JO and Idaehor J O. “Water quality assessment and prevalence of water-related diseases in rural communities of Edo State, Nigeria”. Journal of Applied Sciences and Environmental Management4 (2019): 687-694.
40. Page MI. “The chemistry of β-lactams”. Springer Science and Business Media (2012).
41. Purrello S M., et al. “Methicillin-resistant Staphylococcus aureus infections: A review of the currently available treatment options”. Journal of Global Antimicrobial Resistance 4 (2014): 51-61.
42. Schwarz S., et al. “Lincosamides, pleuromutilins and chloramphenicol: mode of action and mechanisms of resistance”. Cold Spring Harbor Perspectives in Medicine11 (2016): a027037.
43. Seth R., et al. “Water quality evaluation of Himalayan Rivers of Kumaun region, Uttarakhand, India”. Applied Water Science2 (2014): 91-102.
44. Shaw L N., et al. “The role and regulation of the extracellular proteases of Staphylococcus aureus”. Microbiology1 (2004): 217-228.
45. Stojanov M., et al. “Methicillin-resistant Staphylococcus aureus strains isolated in a Croatian hospital show clonal relationship with epidemic strains described in Central Europe”. European Journal of Clinical Microbiology & Infectious Diseases, 31.6 (2012): 1363-1367.
46. Toh S M., et al. “Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to clarithromycin”. Clinical Infectious Diseases12 (2007): 1573-1579.
47. Vaishali P and Punita P. “Assessment of seasonal variation in water quality of River Mini, at Sindhrot, Vadodara”. International Journal of Environmental Sciences5 (2013): 1424-1436.
48. Walsh C. “Antibiotics: actions, origins, resistance”. ASM Press (2016).
49. Walsh C and Wencewicz T. “Prospects for new antibiotics: a molecule-centered perspective”. Journal of Antibiotics6 (2016): 419-425.
50. Weigel L M., et al. “Genetic analysis of a high-level vancomycin-resistant isolate of Staphylococcus aureus”. Science5650 (2003): 1569-1571.
51. “Guidelines for drinking-water quality (4^th ed.)”. World Health Organization (2018).
52. Wiley BB., et al. “Identification of Staphylococcus aureus”. In Manual of clinical microbiology. (9^th ed) (2008): 390-404.
53. Wilson DN. “Ribosome-targeting antibiotics and mechanisms of bacterial resistance”. Nature Reviews Microbiology1 (2014): 35-48.
54. Woodford N. “Biological counterstrike: antibiotic resistance mechanisms of Gram-positive cocci”. Clinical Microbiology and Infection3 (2005): 2-21.

Citation

Citation: Osunla Ayodeji Charles. “Snapshot Survey and Antibiotic Profiles of Staphylococcus Species from Selected Wells in Akungba-Akoko".Acta Scientific Microbiology 8.8 (2025): 27-33.

Copyright

Copyright: © 2025 Osunla Ayodeji Charles. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.