Asma A Elamari¹*, Nawal M Buzgaia², Tahani M Awin², Wafa S Zubi¹, Fawzia Mufthah Aljazwia¹, Aida MM Elakeili³ and Ghazala A Ashour²

¹Botany Department, Science Faculty, University of Benghazi, Benghazi, Libya
²Chemistry Department, Science Faculty, University of Benghazi, Benghazi, Libya
³Biology Department Education Faculty, University of Benghazi, Benghazi, Libya

*Corresponding Author: Asma A Elamari, Botany Department, Science Faculty, University of Benghazi, Benghazi, Libya.

Received: June 02, 2025; Published: June 20, 2025

Abstract

The discovery of antimicrobial agents from natural products remains a primary focus for many pharmaceutical scientists seeking to overcome antibiotic resistance. Ephedra is one of the commonly used plants in traditional medicine. This study aimed to evaluate the antibacterial activity of leave extracts from Ephedra alata against standard laboratory strains and clinical bacterial isolates; including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC8739), Staphylococcus aureus, Escherichia coli, and Klebsella pneumonia, using well diffusion method. 50 μL of Ephedra alata leave extracts prepared in methanol and chloroform solvents and were applied at different concentrations (100, 50, 25, and 12.5 mg/ml), to evaluate their efficacy as antibacterial agent against bacterial growth. The results showed that methanol extracts exhibited high inhibitory activity against both standard and clinical strains of Staphylococcus aureus at all tested concentrations. Methanol extracts also was effective against the growth of Enterococcus faecalis (ATCC 29212) but only at the highest concentration (100 mg/ml). Chloroform leave extracts had inhibitory effect against a clinical isolates of Escherichia coli at high concentrations (50 and 25mg/ml) and Klebsella pneumonia at all applied concentrations. Both methanol and chloroform plant extracts did not exhibit any effect on standard Escherichia coli. This study confirmed the efficacy of Ephedra alata leave extracts as natural antibacterial products and recommended that they may be used as a therapy.

Keywords: Ephedra; Extract; Antibacterial; Activitys

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Citation

Citation: Asma A Elamari.,et al. “Antibacterial Activity of Ephedra alata Extracts: In Vitro Evaluation Against Standard and Clinical Human Pathogenic Bacteria".Acta Scientific Microbiology 8.7 (2025): 29-36.

Copyright

Copyright: © 2025 Asma A Elamari.,et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.