Dr Ramesh Londonkar1* and Maithilee Kesralikar2
1Professor, Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India 2Ph.D. Research Scholar, Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India
1Professor, Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India
2Ph.D. Research Scholar, Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India
*Corresponding Author: Ramesh Londonkar, Professor, Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, India.
Received: October 11, 2022; Published: October 31, 2022
In therapeutic plants, phytochemicals are found in abundance. A family of verbena known as vervain (verbenaceae) consists of over 100 genera and nearly 2600 species. Among the Verbenaceae family is Stachytarpheta, a green perennial herb. This species is being examined in this study for its phytochemical, antioxidant, and anti-inflammatory properties. Stachytarpheta mutabilis methanol extracts were studied for their phytochemical screening, antimicrobial, anti-inflammatory, and anticancerous effects in the current study. Phytochemical study revealed the presence of alkaloids, flavonoids, glycosides, steroids, tannins, carbohydrates, and saponins, among other phytoconstituents. One fungus, Aspergillus niger, Staphylococcus aureus, and Bacillus subtilis, as well as four gram (+) pathogens, including Staphylococcus aureus and Bacillus subtilis, and two gram (-) pathogens, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae, were performed by disc diffusion tested for antibacterial activity .The nitric oxide scavenging assay was used to test for anti-inflammatory efficacy in vitro. DPPH, ABTS, and total antioxidant tests were conducted. Analyses such as TLC, GC-MS, and were performed as per common procedure. A nitric oxide scavenging experiment was utilised to determine the anti-inflammatory properties of the drug at in vitro. Tests for antioxidant activity was conducted using DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) , and total antioxidant tests. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) analysis has been used to do anticancer screening. Extracts from Stachytarpheta mutabilis were found to consist flavonoids, tannins and phenolic compounds as well as terpenoids and steroids in their phytochemical analysis. Squalene, octadecanal, and butan-1-one were found in the GC-MS study indicated other bioactive chemicals such as 4-isopropylcinnamic acid and octadecanoic acid. The methanolic fractions of plant extracts were shown to be effective against all bacteria. Anti-inflammatory and antioxidant experiments were performed in the lab revealed potent inhibition activity of the compound. Analysis of the GC-MS data has revealed the existence of thirty distinct components. MTT assay on the extract indicated anticancer activities of The data shows that the methanolic extract has a significant properties. Stachytarpheta mutabilis has therapeutic value and other pharmaceutical qualities may be assessed as a result of these results, which support the traditional usage of folk medicine as well.
Keywords: Medicinal Plants; Phytochemical Analysis; Antimicrobial; Anti-Inflammatory; Anticancer
Citation: Dr Ramesh Londonkar and Maithilee Kesralikar “Phytochemical and In Vitro Anticancer Activities of Methanolic Extract of Stachytarpheta mutabilis". Acta Scientific Microbiology 5.11 (2022): 85-94.
Copyright: © 2022 Dr Ramesh Londonkar and Maithilee Kesralikar. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.