Abstract

  Total eight soil samples were taken from Sangli district (Maharashtra) India. The potent isolates was identified by using morphological, cultural, biochemical, physiological and 16S rRNA gene sequence analysis. Keratinase production was optimized using different parameters. The 120 hrs incubation period of was observed for optimum keratinolytic protease production. Inoculum age of 10 days was found to be effective in terms of protease production and degradation. 3% inoculum size was showed optimum degradation and proteolytic activity. The agitation rate at 160 rpm was found to be optimum. Optimum enzyme production and feather degradation was found at pH 9.0. Maximum production of protease was observed at 40⁰C. The optimum amount of keratinase was produced by Streptomyces coelicoflavus in presence of dextrose and peptone as a carbon and nitrogen source separately respectively. 1% feather concentration was found optimum keratinase production. Streptomyces coelicoflavus was enhanced feather degradation in the presence of KH₂PO_4.

Keywords: Streptomyces coelicoflavus, 16S rRNA, Keratinase, Optimization and Degradation.

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Citation

Citation: Tooba Momin., et al. “Optimizing the Fermentation Conditions and Enhancing the Keratinase Production from Streptomyces coelicoflavus". Acta Scientific Microbiology 4.3 (2021): 14-24.

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Copyright: © 2021 Tooba Momin., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.