Abstract

  Tuberculosis still represents a disaster that puts heavy shadow over all human societies. In spite of the availability of effective chemotherapy and vaccine, tuberculosis is a global health concern for both developing and developed countries. A defect in the genes of the immune response is the most acceptable explanation for susceptibility of some individuals and resistance of others to TB. Cytokines play critical role in interactions and integration between the cells of immune system, which leads to effective defense against TB, among cytokines IL-4, which have regulatory role in immune response in Iraqi patients with TB. Seventy-four blood specimens were collected from 74 patients in the Institute of the Tuberculosis and Chest Disease-Basra city, blood specimens also collected from 74 healthy individuals as control. Extracted DNA was amplified using two sets of specific primers for promoter regions of IL-4. Purified amplicons were sequenced and were analyzed using specific software. The genotyping analysis of promoter of IL-4 gene was indicated two alleles C and T, of them C allele is predominant (73%), while T allele is recessive (24%). Although those heterozygous genotypes (CT) were present in patient and healthy control (HC), but the frequency of CT in patients was significantly higher than those of controls. IL-4 promoters carry more than one type of mutations pushing toward increasing susceptibility of some individuals to TB.

Keywords: Interleukin-4; Tuberculosis; Mycobacterium tuberculosis

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Citation

Citation: Mazin S Salman and Awatif H Issa. “Genotyping Study for the Promoter of IL-4 Gene in Iraqi Patients with Tuberculosis". Acta Scientific Microbiology 4.1 (2021): 73-76.

Copyright

Copyright: © 2021 Mazin S Salman and Awatif H Issa. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.