Abstract

Laboratory animals are essential in research. Genetic and health quality control enables reliable and reproducible experimental data. A very common disease in rodent colony facilities, is a parasitosis caused by the etiological agent, species: Syphacia muris, which belongs to the filo: Nematoda, to the family: Oxyuridae, is an intestinal endoparasite. The life cycle of this parasite is direct, (there is no intermediate host) has a cycle of 7 to 9 days. Syphacia muris Las infections can alter a humoral host's response to parasitic antigenic stimuli, and cause a Th2-like immune response with the production of elevated cytokines and induce an autoimmunity mediated by Th2 [1]. Therefore a study was carried out in colonies of rats Wistar strain (adult males and females) of a conventional animal care facility of faculty of medicine of the University of Buenos Aires. A total of 36, 32 y 30 rats were tested. Random samples were taken from animals, by Graham's method. Treatments were: treatment A: Ivermectin administered subcutaneously according to the weight of the animal, every 15 days. Treatment B: Ivermectin administered subcutaneously according to the weight of the animal, each week. In treatment C: Ivermectin was administered orally in drinking water at a concentration of 2ml ivermectin in 500ml of filtered water, for four days and allowed to rest for 3 days, this treatment was repeated for three weeks. Together, spray cage spraying containing an ivermectin solution was performed: 1ml in 250 ml of filtered water. The calculated prevalence rate for each treatment was: treatment A: in the first dose 50% and in the second dose of 75%. In treatment B: a 75% rate was obtained at the first and second doses of 80%. In treatment C: it was 0% in the first and second doses. These results enabled the finding of adequate and effective treatment for the eradication of oxyides. A parasitosis very common and common in all biotheres, but so untreated. Therefore, this work demonstrated that it is possible to be treated, eradicated and controlled to avoid interference and alter experimental responses. However, ensure more reliable results by controlling some variables, such as the health status of animals. Obtaining a good experimental model, applying the uses of 3Rs [10], in terms of reduction when treating infected animals and avoiding the subsequent use of other animals and in refinement when ensuring the welfare of animals.

Keywords: Syphacia muris; Wistar Rats; Ivermectin and Conventional Animal Care Facility

References

1. Cortez. A., et al. "Study of the immune response against Syphacia muris in Wistar rat". Biomedica Magazine 31 ( 2011): 209-421.
2. Garfias C. “Parasitic helminths of veterinary concern: host immune response regulation and potential use for the treatment of inflammatory diseases”. Revista Veterinaria México 40 (2009).
3. Cardoso de Martínez C., et al. "The animal as an experimental subject. Technical and ethical aspects. Interdisciplinary Center for Studies in Bioethics". University of Chile (2007).
4. Molina M. "Characterization of biotheres used in scientific research" . Bachelor's thesis. University of Costa Rica. (2002): 1-132.
5. Diaz M. et al. “Pharmacology of endectocides: therapeutic use (II)”. Revista Veterinaria 16. (2000): 15-40.Murcia -España.
6. Pohlmeyer-Esch G. “Implications of infectious agents on results of animal experiments”. Laboratory Animal 1 (1999): 39-87.
7. Lionel Zener. “Effective eradication of pinworms (Syphaciamuris, Syphacia obvelata and Aspiculuris tetraptera) from a rodent breeding colony byoral anthelmintic therapy”. Laboratory Animals 32. (1998): 337-334.
8. Oldham, J. “Helminths, ectoparasites, and protozoa in rats and mice”. In Pathology of laboratory rats and mice”. (1967): 641-679.
9. Martinez C., et al. "Intestinal worms in rodents". The international Journal of Laboratory Animal Science and Welfare 10 (1976): 1-11.
10. Russell W and Burch R. “The principles of human experimental technique”. London (1959).

Citation

Citation: Maria Alfonsina Lizárraga. “Presence of Syphacia muris in Wistar Rats of a Conventional Animal Care Facility and Treatment for the Eradication of this Parasitosis". Acta Scientific Microbiology 3.9 (2020): 69-72.

Copyright

Copyright: © 2020 Maria Alfonsina Lizárraga. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.