Abstract

  Phage therapy has been proposed as a new strategy in aquaculture against pathogen bacteria, especially against antimicrobial resistant strains. It is target specific, and promises to be fast, flexible, and inexpensive. However, it requires a deep characterisation of experimental phages before use. For that reason, many researches have focused on the effect of different phages on aquaculture fish. In contrast, to date, the effect of phage in the human bacteriome has not been studied. Zebrafish (Danio rerio) shares homologies with human in terms of organs and cell types. For that reason, the use of this animal model has increased in recent years. The aim of this report was to study the effect of the AS-A phage, highly active against fish pathogens, on the immunity and intestinal microbiota of a human-conventionalised zebrafish model. The expression level of several immune system associated genes was evaluated by real-time qPCR to achieve this aim. In addition, the microbial population was characterised by 16S sequencing after an over-therapeutic phage exposure for 24 hours. Results showed that AS-A phage did not stimulate zebrafish immune response. However, the bacterial population structure was affected. This research provides evidences of the impact of AS-A from fish to human wellbeing.

Keywords: Phage Safety; Conventionalised Zebrafish; Aquaculture; Animal Model

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Citation

Citation: Nerea Arias-Jayo., et al. “Aquaculture Phage AS-A: Effects on Zebrafish Conventionalised with Human Microbiota". Acta Scientific Microbiology 3.4 (2020): 129-136.

Copyright

Copyright: © 2020 Nerea Arias-Jayo., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.