Assessing the Impact of Stress on the Objectivity of Dental Diagnostic Indicators
MT Alexandrov*
Department of Dentistry, FUV GBUZ MO, Moscow Regional Research Clinical
Institute, Russia
*Corresponding Author: MT Alexandrov, Department of Dentistry, FUV GBUZ MO,
Moscow Regional Research Clinical Institute, Russia.
Received:
June 18, 2026; Published: July 14, 2026
Background. Information on objective data and reference indicators characterizing the condition and relationship of maxillofacial organs (MFO) with general adaptation syndrome and stress is poorly understood. The aim of this study is an experimental and clinical investigation of the objectivity of a method for digital, comprehensive, and express diagnostics of students' dental status under stressful conditions based on metabolic, morphometric, and functional indicators of maxillofacial tissues.
Material and Methods. The study involved 30 students aged 18 to 21 years (17 females and 13 males) before and after mentally challenging classes, after physical exercise, after the end of the first year of study, and at the beginning and middle of the first semester of the second year of study. Electroodontodiagnostics, pH-metry and fluorescence diagnostics of saliva, thermometry of periodontal tissues, Stange test, calculation of the Sheikh-Zade formula, and the Spielberger psychological test were carried out. Statistical analysis of the results was performed using standard statistical complexes in Microsoft Excel.
Results. EOD: after two classes with mental stress - an increase in the indicator by 46.5% (p < 0.01). After physical unloading - recovery by 23.2% (p < 0.05). pH-metry of saliva: at the end of the academic year - 6.41 ± 0.19, after two months of summer vacation - 6.87 ± 0.11, and in the middle of the first semester of the second year - 6.63 ± 0.11. A comparative analysis of periodontal tissue thermometry readings in students at the end of the academic year and after a two-month rest revealed an average increase in gum temperature of 0.62 °C (p < 0.05). Measuring the fluorescence intensity of saliva samples before and after academic stress compared to baseline revealed a significant increase in the 600-650 nm wavelength range and a 10-30-fold increase in integral intensity, while a 7-10-fold decrease was observed after a night's rest. Stange's test revealed a 31.5% decrease (p < 0.001) after two mentally challenging sessions and a 19.3% improvement (p < 0.001) after physical stress. Sheikh-Zadeh formula: after two sessions with mental stress - an increase of 57.2% (p < 0.001), after physical unloading - dynamics to decrease by 18.6% (p < 0.05). Spielberger psychological test after summer vacation: prevalence of the average level of personal anxiety and a high level of reactive anxiety.
Conclusion: the specified examination methods can reasonably be included in a comprehensive assessment of the dental status with the intensification of their correction for the stress factor. The presented indicators of the maxillofacial region objectively and multifactorially reflect the dental status of the maxillofacial region and can be used as reference in assessing stress loads in students at the stages of their training. Key words: stress; reference indicators of stress; maxillofacial region; saliva; fluorescence. For citation: Alexandrov M.T.
Keywords: Maxillofacial Organs (MFO); Metabolic; Saliva
References
- Sel’e G. “Essays on the adaptation syndrome”. Moscow: Medgiz; (1960).
- Sel’e G. “Stress without distress”. Moscow: Meditsina; (1992): 104-109.
- Aleksandrovsky Yu. A. “Borderline mental disorders”. Moscow: Meditsina; (2000).
- Gorizontov PD., et al. “Stress and blood system”. Moscow: Meditsina; (1988): 5-79.
- Efanov OI and Dzanagova TF. “Physiotherapy of dental diseases”. Moscow: Meditsina; (1980): 41-46.
- Marishchuk VL and Evdokimov VI. “Behavior and self-regulation of a person under stress”. St. Petersburg: Izdatel’skiy dom “Sentyabr”; (2001): 52-60, 81-85.
- Meerson FZ. “Adaptation of the body to stressful situations and the prevention of cardiac arrhythmias”. Uspekhi Fiziologicheskikh Nauk4 (1987): 56-79.
- Strygin KN and Poluektov MG. “Modern ideas about stress and the protective role of sleep”. Meditsinskiy Sovet 5 (2015): 70-76.
- Averina AO and Fedoseeva VI. “The study of the manifestations of academic stress in the lives of students”. Scientific and Methodological Electronic Journal “Kontsept” 46 (2016): 8-12.
- Shemyakina OO. “Analysis of the causes of stress and methods for its prevention”. Psychology and Law 2 (2012): 122-132.
- Shcherbatykh Yu V. “Examination and student health. Higher education in Russia”. 3 (2000): 111-115.
- Shcherbatykh Yu V. “Psychology of stress and correction methods”. St. Petersburg: Peter; (2006).
- Bodrov VA. “Cognitive processes and psychological stress”. Psychological Journal4 (1996): 64-74.
- Aghajanyan N A. “Stress and adaptation theory”. Orenburg: IPK GOU OGU; (2005): 60-94.
- Sudakov K V. “Individual resistance to stress”. Moscow; (1998): 3-268.
- Aleksandrov MT. “Laser clinical biophotometry (theory, experiment, practice)”. Moscow: Tekhnosfera; 2008.
- Aleksandrov MT., et al. “The study of the spectral characteristics of the pelvic organs in women and their clinical significance”. Onkoginekologiya 3 (2013): 63-67.
- Bilinsky II., et al. “Changes in the biochemical properties of saliva and their effect on the dental status of students under the influence of stress”. Naukovi Pratsi VNTU 1 (2017): 1-6.
- Block B. “Levels of wakefulness and attention. Experimental psychology”. Moscow: Progress; 3 (2010): 7-146.
- Danilevskiy MF and Borisenko AV. “Periodontal disease”. Kiev: Health; (2000).
- Zabolotsky IB and Ilyukhina VA. “The physiological basis of the differences in stress resistance of a healthy and sick person”. Krasnodar: Publishing House of the Kuban Medical Academy; (1995).
- Korkushko OV. “Cardiovascular system and age (clinical and physiological aspects)”. Moscow: Meditsina; (2003).
- Sheikh-Zade YuR., et al. “Determination of the proper heart rate in a person at rest”. Human Fiziology6 (2001): 114-116.
- Ustyuzhaninova NV. “Functional state of external respiration of healthy students”. Byull SO RAMS 1 (2004): 134-137.
- Batarshev AV. “Psychology of personality and communication”. Moscow: Humanitarian Publishing House VLADOS; (2003).
- Danilova NN and Krylova AL. “Physiology of Higher Nervous Activity”. Rostov n/D: Feniks; (2002): 406-7.
- Erofeeva LM., et al. “Structural and functional characteristics of the pulp of the teeth of rats under conditions of emotional and cold stress”. Morphology. St. Petersburg: Eskulap 137.4 (2010): 74.
- Manuylova EV and Mikhalchenko VF. “Modern problems of science and education”. 2 (2013): 115-118. Gorenkov RV., et al. “Chronic hypoxia as one of the factors of increased fluorescence of endogenous porphyrins in living biological tissues”. Biofizika 4 (2007): 711-717.
- Rogatkin DA. “Laser medicine”. [Lazernaya meditsina] 4.1 (2000): 30-35.
- Aleksandrov MT., et al. “Laser Raman-fluorescence medical technologies in dentistry: from experiment to clinic”. Moscow, (2020): 348.
- Method for assessing the adaptability and quality of dental treatment, patent RU23702210C1.
- Aleksandrov MT., et al. “Method and Device for Raman Fluorescence Diagnostics of the State of Human Tissues in Health and Pathology”. Acta Scientific Microbiology4 (2025): 49-58.
- Aleksandrov MT., et al. “Conceptual Justification of the use of Raman-Fluorescent Medical Technologies for A Comprehensive Assessment of the Hygienic Condition of the Oral Cavity”. Acta Scientific Dental Sciences4 (2025): 40-49.
- Alexandrov MT., et al. “Features of mineralization of hard tissues of the tooth in carious and non-carious lesions”. Journal of Dental Health Oral Research1 (2025): 1-9.
- Huling LB., et al. “Study Summary. The effects of stress on general and oral health are increasingly recognized”. Journal of Oral Pathology and Medicine 41 (2012): 149-152.
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