Riaz Roohi¹, Suresh Anand²*, Ganesh Arathi³, Nagendrababu Venkateshbabu³ and Deivanayagam Kandaswamy³

¹Conservative Dentistry and Endodontics, Sri Ramachandra University, India
²Dental Director, Taunton Dentistry and Implants PC, United States
³Conservative Dentistry and Endodontics, Sri Ramachandra University, India

*Corresponding Author: Suresh Anand, Dental Director, Taunton Dentistry and Implants PC, United States.

Received: November 20, 2024; Published: December 03, 2024

Abstract

Introduction: Adherence of bacteria to a substrate is the first stage in biofilm formation. This investigation aimed to study the ef fects of final Irrigants on the force of adhesion of E. faecalis to dentin by using atomic force microscopy. This information could aid in understanding the force on interaction between E. faecalis and dentin and subsequently facilitate in designing treatment strategies which would prevent further bacterial recolonization in the root canal.

Methods: The irrigants tested in our study were 1.3% NaOCl, 1.3% NaOCl + 17% EDTA, 1.3% NaOCl + MTAD, 1.3% NaOCl + 18% HEBP and untreated samples (control). The force of adhesion was measured by using atomic force microscopy. One way ANOVA fol lowed by Post Hoc multiple comparision Tukey HSD tests were used to analyze the data.

Results: There was a significant increase in the adhesion force after irrigation of dentin when EDTA and HEBP were used as the final irrigants whereas the NaOCl-treated dentin was similar to the control group.

Conclusion: With the use of MTAD as a final irrigant, a repulsive force was seen between E.faecalis and dentin. This study highlighted that chemicals that alter the physicochemical properties of dentin will influence the nature of adhesion force and subsequent biofilm formation of E. faecalis to dentin.

Keywords: Adhesive Force; Repulsive Force; Atomic Force Microscopy; MTAD; HEBP; EDTA; NaOCl

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Citation

Citation: Suresh Anand., et al. “AInfluence of Various Irrigating Regimens on the Force of Adhesion of Enterococcus Faecalis to Root Dentin using Atomic Force Microscopy - An In Vitro Study".Acta Scientific Dental Sciences 9.1 (2025): 02-08.

Copyright

Copyright: © 2025 Suresh Anand., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.