Acta Scientific Dental Sciences (ISSN: 2581-4893)

Research ArticleVolume 5 Issue 6

The Effect of Warm Gutta-Percha Techniques on Viability and Mineralized Tissue Associated Gene’s Transcripts of Human Periodontal-Ligament Mesenchymal Stem-Cells

Nazife Tuğba Azmaz1, Serife Buket Bozkurt2, Sema Sezgin Hakki3 and Sema Belli4*

1Specialist Endodontist, Meram Research and Training Hospital, Konya, Turkey

2Research Lab of Faculty of Dentistry, Hacettepe University, Ankara, Turkey

3Full-Time Professor in Department of Periodontology, Selcuk University, Konya, Turkey

4Full-Time Professor in Department of Endodontics, Selcuk University, Konya, Turkey

*Corresponding Author: Sema Belli, Full-Time Professor in Department of Endodontics, Selcuk University, Konya, Turkey.

Received: March 20, 2021; Published: : May 05, 2021

Citation: Sema Belli., et al. “The Effect of Warm Gutta-Percha Techniques on Viability and Mineralized Tissue Associated Gene’s Transcripts of Human Periodontal-Ligament Mesenchymal Stem-Cells". Acta Scientific Dental Sciences 5.6 (2021): 11-20.

Abstract

Aim: The purpose of the this study was to assess the effect of continuous wave of condensation (CWCT) and thermoplastic gutta-percha injection (TGI) techniques on the viability and mRNA expressions of mineralized tissue-associated proteins of human periodontal-ligament mesenchymal stem-cells (hPDL-MSCs).

Methods: hPDL-MSCs were isolated from premolar-teeth. Roots of human premolar-teeth were placed vertically to the cell-culture dishes. Six groups were designed as follows: control 1 (without teeth, C1); control 2 (with teeth C2); AH Plus group (AH); single-cone obturation group (SC); CWCT and TGI. The viability of the hPDL-MSCs was analyzed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide at 24 h and 96h. RNA was isolated from the hPDL-MSCs at 24 hrs and cDNA synthesis was performed. The mRNA expression of mineralized tissue-markers including bone sialoprotein (BSP), osteocalcin (OCN), runt-related transcription factor-2 (Runx2), type I collagen (COL I), alkaline phosphatase (ALP), were evaluated by quantitative real time polymerase chain reaction (qRT-PCR).

Results: Reduced cell viability was observed in all groups except the controls. When SC and CWCT and TGI groups were compared, it was observed that heat had a significant negative effect on cell viability (p < 0.05). Reduction in the mRNA expressions of BSP, OCN, and ALP were observed at the test groups and this negative effect was higher in the heat treated groups (p < 0.05).

Conclusion: Warm gutta-percha techniques reduced mRNA expressions of mineralized tissue-associated proteins which are critical for the functions of PDL-MSCs during healing at the apical region after endodontic treatment.

Keywords: Associated Proteins; Mineralized Tissue; Periodontal Ligament; Mesenchymal Stem Cells; Warm Gutta-Percha Techniques

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Copyright: © 2021 Sema Belli., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



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