Acta Scientific Dental Sciences (ASDS)(ISSN: 2581-4893)

Research Article Volume 5 Issue 2

Estimation of Serum Copper Levels in Oral Submucous Fibrosis Patients: A Quantitative Study

Bhavna Tyagi1*, Deepak Bhargava1, Ritika Sharma1,Vidyadevi Chandarvarkar1 and Pradeep Sundersan2

1Oral and Maxillofacial Pathology and Microbiology, Sharda University, India
2Ph.D (Education), American University, London, UK

*Corresponding Author: Bhavna Tyagi, Oral Pathologist, Oral and Maxillofacial Pathology and Microbiology, Sharda University, Greater Noida, Uttar Pradesh, India.

Received: December 14, 2020; Published: January 23, 2021



Context: Oral submucous fibrosis (OSMF) is documented as a potentially malignant disorder with a 7.6% malignant transformation rate. Various trace elements such as, copper, zinc, and iron are getting much attention in the early recognition of cancer and potentially malignant disorders including OSMF.

Aim: To evaluate the levels of copper in the OSMF patients’ blood serum and compare with healthy individuals.

Settings and Design: Quantitative research was conducted in the Department of Oral and Maxillofacial Pathology, Sharda University, Greater Noida. A total of 80 participants, out of which 20 participants were case-control (Batch I), and 60 diagnosed OSMF patients (cases) (Batch II, III and IV) participated.

Methods and Material: For estimation of serum copper, under sterile conditions, 3ml of the venous blood sample was collected. Serum was collected and evaluated by adding an instructed proportion of buffer, and the color reagents in the clean glassware.

Statistical Analysis: All the data was systematically tabulated and analyzed using SPSS statistical software by employing “One way ANOVA”.

Results: The mean serum copper (µg/dl) level differed sig­nificantly (p < 0.004) between all the batches. Mean serum copper (µg/dl) levels in Batch I, Batch II, Batch III, Batch IV was 103.43 ± 5.88603, 105.1 ± 5.99034, 121.55 ± 8.08817, 134.825 ± 18.23334, respectively.

Conclusion: Serum copper levels can be used as a potential diagnostic and prognostic marker in the patient with OSMF condition.

Keywords: Oral Submucous Fibrosis; Trace Elements; Copper; Pre-Cancer; Diagnostic Marker; Serum Copper



  1. Pindborg Jens J and Satyavati M Sirsat. "Oral submucous fibrosis”. Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology6 (1966): 764-779.
  2. Sachdev Prageet K., et al. "Zinc, copper, and iron in oral submucous fibrosis: A meta-analysis”. International Journal of Dentistry 2018 (2018).
  3. Dey Dipten., et al. "Evaluation of salivary copper and zinc levels in oral submucous fibrosis patients”. International Journal of Health and Allied Sciences 2 (2016): 35-37.
  4. Trivedy C., et al. "The upregulation of lysyl oxidase in oral submucous fibrosis and squamous cell carcinoma”. Journal of Oral Pathology and Medicine6 (1999): 246-251.
  5. Wang Tong-Hong., et al. "Lysyl oxidase and the tumour microenvironment”. International Journal of Molecular Sciences1 (2017): 62.
  6. More CB., et al. "Classification system for oral submucous fibrosis”. Journal of Indian Academy of Oral Medicine and Radiology1 (2012): 24.
  7. Messadi DV. “Diagnostic aids for detection of oral pre-cancerous conditions”. International journal of Oral Science 2 (2013): 59-65.
  8. Shah N. “Oral cancer in India: Aetiological factors and prevention”. Journal of the Indian Dental Association 3 (1989): 3-6.
  9. Yadav A., et al. "Estimation of serum zinc, copper, and iron in the patients of oral submucous fibrosis”. National Journal of Maxillofacial Surgery2 (2015): 190.
  10. Maher R., et al. "Role of areca nut in the causation of oral submucous fibrosis: A case-control study in Pakistan”. Journal of Oral Pathology and Medicine 23 (1994): 65-69.
  11. Hazarey VK., et al. "Oral submucous fibrosis: Study of 1000 cases from central India”. Journal of Oral Pathology and Medicine 36 (2007): 12-17.
  12. Kumar LB., et al. "Evaluation of mast cells and burning sensation in various stages of Oral Submucous Fibrosis”. Journal of Oral Biology and Craniofacial Research4 (2020): 430-434.
  13. Srivastava R., et al. "Prevalence of oral submucous fibrosis in patients visiting dental OPD of a dental college in Kanpur: A demographic study”. Journal of Family Medicine and Primary Care8 (2019): 2612.
  14. Zaidi JH., et al. "Radiochemical neutron activation analysis for trace elements of basic ingredients of pan”. Journal of Radioanalytical and Nuclear Chemistry3 (2002): 459-64.
  15. Desai VD., et al. "Molecular analysis of trace elements in oral submucous fibrosis and future perspectives”. Universal Research Journal of Dentistry1 (2014): 26-35.
  16. Shetty SR., et al. "Role of serum trace elements in oral precancer and oral cancer-a biochemical study”. Cancer Research and Treatment 1 (2013): 1-3.
  17. Balpande AR and Sathawane RS. “Estimation and comparative evaluation of serum iron, copper, Zinc and copper/zinc ratio in oral leukoplakia, submucous fibrosis and squamous cell carcinoma”. Journal of Indian Academy of Oral Medicine and Radiology 2 (2010): 73.
  18. Tadakamadla J., et al. "Evaluation of serum copper and iron levels among oral submucous fibrosis patients”. Medicina Oral, Patología Oral y Cirugía Bucal 7 (2011): e870-873.
  19. Varghese I., et al. "Serum copper and zinc levels in pre-malignant and malignant lesions of the oral cavity”. Oncology 4 (1987): 224-227.
  20. Anuradha CD and Devi CS. “Studies on the hematological profile and trace elements in oral submucous fibrosis”. Journal of Clinical Biochemistry and Nutrition1 (1995): 9-17.
  21. Kode MA and Karjodkar FR. “Estimation of the serum and the salivary trace elements in OSMF patients”. Journal of Clinical and Diagnostic Research: JCDR6 (2013): 1215.
  22. Pillai KG and Burde KN. “Increased copper level in oral mucosal tissue of patients with submucous fibrosis and who chew areca nut products”. West Indian Medical Journal4 (2005): 270-271.
  23. Yang YH., et al. "Epidemiological survey of oral submucous fibrosis and leukoplakia in aborigines of Taiwan”. Journal of Oral Pathology and Medicine 4 (2001): 213-219.
  24. Hosthor SS., et al. "Quantitative analysis of serum levels of trace elements in patients with oral submucous fibrosis and oral squamous cell carcinoma: A randomized cross-sectional study”. Journal of Oral and Maxillofacial Pathology: JOMFP 1 (2014): 46.
  25. Nasulewicz A., et al. "Role of copper in tumour angiogenesis—clinical implications”. Journal of Trace Elements in Medicine and Biology 1 (2004): 1-8.
  26. Jayadeep A., et al. "Serum levels of copper, Zinc, iron and ceruplasmin in oral leukoplakia and squamous cell carcinoma”. Journal of Experimental and Clinical Cancer Research: CR3 (1997): 295-300.


Citation: Bhavna Tyagi.,et al. “Estimation of Serum Copper Levels in Oral Submucous Fibrosis Patients: A Quantitative Study”. Acta Scientific Dental Sciences 5.2 (2021): 99-104.


Acceptance rate33%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US