Abstract

Aim of the Work: The aim of the study was to evaluate the effect of enlargement of the apical one third of root canals; by using F4 in comparison to F3 Protaper files; on the amount of Enterococcus Faecalis and surface irregularities in the apical third.

Materials and Methods: 24 freshly extracted human single canalled premolars were obtained. The root lengths were standardized to 15 ± 1 mm. The root canals were instrumented up to #20 K-file till the apical constriction for standardization. Following root canal preparation and sterilization, all root canals were completely filled with 30µ E. faecalis suspension and incubated at 37°C for one week with refreshment every 48 hours. All teeth were sampled after bacterial injection and before instrumentation for examination of bacterial presence inside the canals. For the apical one third assessment, Scanning Electron Microscope "SEM" was used to examine the surface irregularities.

Results: The results concerning bacterial reduction revealed that Group II (F4), showed a significantly higher E. faecalis reduction than Group I (F3) (P = 0.025). However, none of the preparation sizes rendered a bacterial free root canal system. Topographic surface analysis revealed that group II (F4) had significantly lower surface irregularities in comparison to group I (F3) (P = 0.036).

Conclusion: Within the limitations of this study, it could be concluded that none of the root canal preparation sizes resulted in complete E. faecalis elimination. Bacterial reduction has a direct relation with the enlargement of the mechanical preparation. The larger the file size the more significant is the bacterial reduction. This result might be related to the significant reduction in surface irregularities.

Keywords: Enterococcus faecalis; Surface Irregularities; Scanning Electron Microscope; Colony Forming Unit

References

1. Tabassum Sadia and Farhan Raza Khan. “Failure of Endodontic Treatment: The Usual Suspects”. European Journal of Dentistry1 (2016): 144-147.
2. Navabi Amir Arsalan., et al. “Comparative Evaluation of Enterococcus Faecalis Counts in Different Tapers of Rotary System and Irrigation Fluids: An Ex Vivo Study”. Dental Research Journal3 (2018): 173.
3. Rodrigues Renata Costa Val., et al. “Influence of the Apical Preparation Size and the Irrigant Type on Bacterial Reduction in Root Canal-Treated Teeth with Apical Periodontitis”. Journal of Endodontics7 (2017): 1058-1063.
4. Aminoshariae A and JC Kulild. “Master Apical File Size-Smaller or Larger: A Systematic Review of Healing Outcomes”. International Endodontic Journal7 (2015): 639-647.
5. Prati Carlo., et al. “Appearance of the Root Canal Walls after Preparation with NiTi Rotary Instruments: A Comparative SEM Investigation”. Clinical Oral Investigations2 (2004): 102-110.
6. Abou‐Rass M and G. Bogen. “Microorganisms in Closed Periapical Lesions”. International Endodontic Journal1 (1998): 39-47.
7. Akpata E S and H Blechman. “Bacterial Invasion of Pulpal Dentin Wall in Vitro”. Journal of Dental Research2 (1982): 435-438.
8. Baumgartner J Craig., et al. “Association of Black-Pigmented Bacteria with Endodontic Infections”. Journal of Endodontics6 (1999): 413-415.
9. Munson M A., et al. “Molecular and Cultural Analysis of the Microflora Associated with Endodontic Infections”. Journal of Dental Research11)2002): 761-766.
10. Ørstavik Dag. “Root Canal Disinfection: A Review of Concepts and Recent Developments”. Australian Endodontic Journal277 (2003): 70-74.
11. Marinho Ariane Cassia Salustiano., et al. “Influence of the Apical Enlargement Size on the Endotoxin Level Reduction of Dental Root Canals”. Journal of Applied Oral Science6 (2013): 661-666.
12. Rollison Scott., et al. “Efficacy of Bacterial Removal from Instrumented Root Canals in Vitro Related to Instrumentation Technique and Size”. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics3 (2002): 366-371.
13. Paraskevopoulou Mary Th and Marouan G. Khabbaz. “Influence of Taper of Root Canal Shape on the Intracanal Bacterial Reduction”. The Open Dentistry Journal1 (2016): 568-574.
14. Siqueira Jr José F., et al. “Mechanical Reduction of the Bacterial Population in the Root Canal by Three Instrumentation Techniques”. Journal of Endodontics5 (1999): 332-335.
15. Mickel André K., et al. “The Role of Apical Size Determination and Enlargement in the Reduction of Intracanal Bacteria”. Journal of Endodontics1 (2007): 21-23.
16. Yadav Sukhwant Singh., et al. “Effect of ‘Apical Clearing’ and ‘Apical Foramen Widening’ on Apical Ramifications and Bacterial Load in Root Canals”. The Bulletin of Tokyo Dental College2 (2014): 67-75.
17. Peters Ove A., et al. “Effects of Four Ni-Ti Preparation Techniques on Root Canal Geometry Assessed by Micro Computed Tomography”. International Endodontic Journal3 (2001): 221-230.
18. De-Deus Gustavo., et al. “Micro-CT Evaluation of Non-Instrumented Canal Areas with Different Enlargements Performed by NiTi Systems”. Brazilian Dental Journal6 (2015): 624-629.
19. Versiani Marco Aurélio., et al. “Micro-Computed Tomography Study of Oval-Shaped Canals Prepared with the Self-Adjusting File, Reciproc, Waveone, and Protaper Universal Systems”. Journal of Endodontics8 (2013): 1060-1066.
20. Singla Mamta., et al. “Comparative Evaluation of Rotary ProTaper, Profile, and Conventional Stepback Technique on Reduction in Enterococcus Faecalis Colony-Forming Units and Vertical Root Fracture Resistance of Root Canals”. Oral Surg, Oral Medicine, Oral Pathology, Oral Radiology and Endodontics3 (2010): 105-110.
21. Moyin Shabna., et al. “An Ex Vivo Evaluation of Antimicrobial Efficacy of Root Canal Preparation with Hand K-Files, Hand Pro Taper, and Pro Taper Rotary Files”. Journal of Pharmacy and Bioallied Sciences2 (2019): 305.
22. Ruddle Clifford J. “Finishing the Apical One Third: Endodontic Considerations”. Dentistry Today5 (2002): 66-73.
23. Figdor David and Malin Brundin. “Contamination Controls for Analysis of Root Canal Samples by Molecular Methods: An Overlooked and Unsolved Problem”. Journal of Endodontics7 (2016): 1003-1008.
24. Srikanth P., et al. “Minimal Apical Enlargement for Penetration of Irrigants to the Apical Third of Root Canal System: A Scanning Electron Microscope Study”. Journal of International Oral Health : JIOH6 (2015): 92-96.
25. Michon Anne-Laure., et al. “Advances toward the Elucidation of Hypertonic Saline Effects on Pseudomonas Aeruginosa from Cystic Fibrosis Patients”. PloS One2 (2014): e90164.
26. Vossoghi Maryam., et al. “Efficacy of a Novel Rotary System in Reduction of Intracanal Bacteria: An in Vitro Study”. Iranian Endodontic Journal3 (2016): 219-222.
27. Akhlaghi Nahid Mohammadzadeh., et al. “The Effect of Size and Taper of Apical Preparation in Reducing Intra-Canal Bacteria: A Quantitative SEM Study”. Iranian Endodontic Journal1 (2013): 61-65.
28. De Carvalho Fredson Marcio Acris., et al. “Cleaning Effectiveness of a Reciprocating Single-File and a Conventional Rotary Instrumentation System”. The Open Dentistry Journal1 (2016): 704-713.
29. Peciuliene V., et al. “Isolation of Yeasts and Enteric Bacteria in Root‐filled Teeth with Chronic Apical Periodontitis”. International Endodontic Journal6 (2001): 429-434.
30. Ørstavik Dag and Markus Haapasalo. “Disinfection by Endodontic Irrigants and Dressings of Experimentally Infected Dentinal Tubules”. Dental Traumatology4 (1990): 142-149.
31. Kreft ÁB., et al. “Aggregation Substance of Enterococcus Faecalis Mediates Adhesion to Cultured Renal Tubular Cells”. Infection and Immunity1 (1992): 25-30.
32. Machado Manoel Eduardo De Lima., et al. “Comparison of Two Rotary Systems in Root Canal Preparation Regarding Disinfection”. Journal of Endodontics7 (2010): 1238-1240.
33. Souza Ronaldo Araújo., et al. “Apical Third Enlargement of the Root Canal and Its Relationship with the Repair of Periapical Lesions”. European Journal of Dentistry4 (2012): 385-388.
34. Dalton B Clark., et al. “Bacterial Reduction with Nickel-Titanium Rotary Instrumentation”. Journal of Endodontics11 (1998): 763-767.
35. Matos Neto M., et al. “Effectiveness of Three Instrumentation Systems to Remove Enterococcus Faecalis from Root Canals”. International Endodontic Journal5 (2012): 435-438.
36. Moshari Amir Abbas., et al. “Reduction of Enterococcus Faecalis in Curved Root Canals after Various Sizes and Tapers of Canal Preparation”. Journal of Conservative Dentistry: JCD4 (2015): 306.

Citation

Citation: Mohammed Yasien Taher Alagha., et al. “Evaluation of the Effect of Enlargement of the Apical One Third by Protaper Universal Rotary Files System on Elimination of Enterococcus faecalis and Surface Irregularities in Single Canaled Premolars (A Comparative In-Vitro Study)”. Acta Scientific Dental Sciences 5.1 (2021): 100-109.

Copyright

Copyright: © 2021 Mohammed Yasien Taher Alagha., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.