Abstract

Introduction: Micro-computed tomography (micro-CT) has become the gold standard for evaluating bone microarchitectures in small animal models. Various studies have employed micro-CT to investigate rat bones. However, results can be significantly influenced by rotation range (RR) and scan resolution (SR).

Objective: Through our study, we tried to assess effects of RR and SR during Micro-computed tomography scan.

Materials and Methods: In this study, nine femurs were scanned based on four different acquisition scenarios using all possible combinations of two SRs (SR: 9 μm/pixel and 18 µm/pixel) and two RRs (RR: 180° and 360°).

Results: The scan durations and file sizes were statistically significantly different between different groups: A-C (p = 0.004; p = 0.002), B-C (p = 0.001; p = 0.001), and B-D (p = 0.004; p= 0.007). No statistically significant differences between groups were observed for the bone volume (p = 0.1589), trabecular number (p = 0.4160), trabecular separation (p = 0.6251), or volume of closed pores (p = 0.0538). In contrast, trabecular thicknesses were statistically significantly different between various groups: A-D (p = 0.03) and B-D (p = 0.043). Furthermore, the cortical bone morphometry corresponding to the number of closed pores was statistically significantly different between various groups: A-C (p = 0.004), A-D (p= 0.001), and B-D (p = 0.011).

Conclusion: SR and RR affect acquisition time, data storage, also the quantitative results of trabecular bone micro-CT assessments.

Keywords: Micro-Computed Tomography; Rotation Range; Scan Resolution; Pixel Size; Trabecular Bone

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Citation

Citation: Simone Peixe Friedrichsdorf., et al. “Micro-Computed Tomography Assessment of Rat Bone Microstructures: Effects of Acquisition Resolution and Rotation Range”. Acta Scientific Dental Sciences 4.6 (2020): 48-53.

Copyright

Copyright: © 2020 Simone Peixe Friedrichsdorf., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.