Acta Scientific Biotechnology

Research Article Volume 3 Issue 6

Diagnosis of Citrus tristeza Virus (CTV) From Mother Plant and Seedling of Poncirus trifoliata (Trifoliate Orange) and Citrus reticulata (Mandarin Orange) and Production of Virus Free Plants Through Tissue Culture

Amrit Jnawali, Binju KC, Biplab Adhikari, Sudha Acharya and Prof Dr Mukunda Ranjit*

Department of Biotechnology, Kantipur Valley College- Purbanchal University , Nepal

*Corresponding Author: Prof Dr Mukunda Ranjit, Department of Biotechnology, Kantipur Valley College- Purbanchal University , Nepal.

Received: September 23, 2022; Published: November 10, 2022


Citrus fruits are one of the most delicious fruits belonging to the family Rutaceae. One of the major problems of Citrus in Nepal is Citrus tristeza Virus (CTV). CTV is the severe disease of sub-tropical crops of Nepal. CTV was diagnosed in different mother plants and seedlings of Poncirus trifoliata and Citrus reticulata. CTV was not transferred from infected mother plants of Trifoliate orange and Mandarin orange to their seedlings if the seeds are grown in in-vitro culture and ex-vivo (in insect proof screen house). DAS-ELISA techniques were used for the diagnosis of CTV from the Citrus plants. The values of <0.2 on ELISA plate reader was considered a negative reference for CTV. CTV infected Trifoliate orange and Mandarin orange seeds were grown in -vitro culture in MS Medium and the media optimization was done for the seedlings obtained from them. The best shoot multiplication, shoot length was found better in 1 mg/l BAP+0.1 mg/l NAA media and similarly best root number and root length was found better on 1 mg/l NAA media.

Keywords: Citrus tristeza Virus (CTV); DAS-ELISA; Poncirus trifoliata; Citrus reticulata


  1. A Gautam., et al. “Economics of production and marketing of mandarin in gulmi, NEPAL”. Food and Agribusiness Management (FABM) 1 (2020): 1-4.
  2. S Dahal., et al. Food and Agribusiness Management (FABM). 1.1 (2020): 47-53.
  3. S Atta., et al. “Distribution and Research Advances of Citrus tristeza virus”. Journal of Integrative Agriculture 3 (2012): 346-358.
  4. M L Edwards and J I Cooper. “Plant virus detection using a new form of indirect ELISA”. Journal of Virological Methods4 (1985): 309-319.
  5. F Chamandoosti. “The utilities of Citrus tissue culture”. International Journal of Agriculture and Environmental Research 9 (2017): 36-46.
  6. “RS5687_Master Plan for Horticulture Development Fruit Production in Nepal-scr”.
  7. P Acharya. “Study on Citrus Tristeza Virus (Ctv) Vectors in a Mandarin Farm of Kavrepalanchowk District , Nepal”. (2017).
  8. N S Poudel and K Khanal. “Viral Diseases of Crops in Nepal”. International Journal of Applied Sciences and Biotechnology2 (2018): 75-80.
  9. P Moreno., et al. “Citrus tristeza virus: A pathogen that changed the course of the citrus industry”. Molecular Plant Pathology 2 (2008): 251-268.
  10. M Alhajj and A Farhana. “Enzyme Linked Immunosorbent Assay”. Treasure Island (FL), (2022).
  11. S Korkmaz., et al. “Detection of Citrus tristeza virus (CTV) from Satsuma Owari mandarins (Citris unshiu) by direct tissue blot immunoassay (DTBIA), DAS‐ELISA, and biological indexing”. New Zealand Journal of Crop and Horticultural Science4 (2008): 239-246.
  12. MA Rocha-Peña and R F Lee. “Serological techniques for detection of citrus tristeza virus”. Journal of Virological Methods3 (1991): 311-331.
  13. TA Thorpe. “History of plant tissue culture.”. Molecular Biotechnology2 (2007): 169-180.
  14. T Murashige. “Plant Propagation Through Tissue Cultures”. Annual Review of Plant Biology 1 (1974): 135-166.
  15. MZ Abdin., et al. “Plant biotechnology: Principles and applications”. March. (2017).
  16. A Hussain., et al. “Plant Tissue Culture: Current Status and Opportunities”. A. Leva and L. M. R. Rinaldi, Eds. Rijeka: IntechOpen, (2012): 1.
  17. Resham Babu Amgai., et al. “Hormonal Effect on Mandarin Orange (Citrus reticulata Blanco)”. Nepal Journal of Biotechnology1 (2016): 33-36.
  18. TM Campus. “Journal of Tikapur Multiple Campus”. 5 (2022): 152-167.
  19. AA Sofian., et al. “Effect of IBA and BAP on Shoot Growth of Tawangmangu Tangerine (Citrus reticulate) by In-Vitro”. Biosaintifika Journal of Biology and Biology Education2 (2018): 379-387.


Citation: Prof Dr Mukunda Ranjit., et al. “Diagnosis of Citrus tristeza Virus (CTV) From Mother Plant and Seedling of Poncirus trifoliata (Trifoliate Orange) and Citrus reticulata (Mandarin Orange) and Production of Virus Free Plants Through Tissue Culture".Acta Scientific Biotechnology 3.6 (2022): 01-12.


Copyright: © 2022 Prof Dr Mukunda Ranjit., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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