Diagnosis of Citrus tristeza Virus (CTV)
From Mother Plant and Seedling of Poncirus trifoliata (Trifoliate Orange) and Citrus reticulata
(Mandarin Orange) and Production of Virus Free Plants Through Tissue Culture
Amrit Jnawali, Binju KC, Biplab Adhikari, Sudha Acharya and Prof Dr Mukunda Ranjit*
Department of Biotechnology, Kantipur Valley College- Purbanchal University ,
*Corresponding Author: Prof Dr Mukunda Ranjit, Department of Biotechnology, Kantipur Valley College- Purbanchal University ,
September 23, 2022; Published: November 10, 2022
fruits are one of the most delicious fruits belonging to the family Rutaceae
. One of the major problems of Citrus
in Nepal is Citrus tristeza
Virus (CTV). CTV is the severe disease of sub-tropical crops of Nepal. CTV was diagnosed in different mother plants and seedlings of Poncirus trifoliata
and Citrus reticulata.
CTV was not transferred from infected mother plants of Trifoliate orange and Mandarin orange to their seedlings if the seeds are grown in in-vitro
culture and ex-vivo
(in insect proof screen house). DAS-ELISA techniques were used for the diagnosis of CTV from the Citrus
plants. The values of <0.2 on ELISA plate reader was considered a negative reference for CTV. CTV infected Trifoliate orange and Mandarin orange seeds were grown in -vitro culture in MS Medium and the media optimization was done for the seedlings obtained from them. The best shoot multiplication, shoot length was found better in 1 mg/l BAP+0.1 mg/l NAA media and similarly best root number and root length was found better on 1 mg/l NAA media.
Keywords: Citrus tristeza Virus (CTV); DAS-ELISA; Poncirus trifoliata; Citrus reticulata
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