Yogesh Kumar*, Daksh Arora and Jhanvi Madan

HCM Agro Products Pvt Ltd, New Delhi, India

*Corresponding Author: Yogesh Kumar, HCM Agro Products Pvt Ltd, New Delhi, India.

Received: February 06, 2025; Published: March 31, 2026

Abstract

Rice (Oryza sativa L.) is a staple food crop for a large part of the global population, and increasing its productivity and growth is essential for ensuring food security. A field study was conducted at Kunjpura, Karnal, Haryana to evaluate the effect of HCM Mycorrhizal, a bioinoculant containing Glomus intraradices, Glomus mosseae, Glomus aggregatum, and Rhizophagus clarus, on paddy growth and productivity. The experiment included two treatments: T1-HCM Mycorrhiza and T2-control with three replications. The study measured AMF colonization, spore count, dry weight, and grain yield at transplanting, tillering, and flowering stages. Results showed that AMF colonization in T1 is 15.2% at transplanting, 29.7% at tillering and 33.4% at flowering, while the control remained very low (1.1–2.4%). Similarly, spore count in T1 noted 10.7 spores/g soil at transplanting, 17.9 at tillering, and slightly decreased to 15.2 at flowering, whereas the control had negligible spore numbers (0–1.0 spores/g soil). Dry weight of treated plants increased from 10.54 g/plant at transplanting to 19.9 g/plant at tillering and 76.8 g/plant at flowering, compared to 6.90–43.12 g/plant in the control. Grain yield was also higher in T1 (2.29 t/acre) than T2 (1.88 t/acre). This indicates the potential of HCM Mycorrhizal as a sustainable and eco-friendly biofertilizer for maintaining long-term productivity and soil health in paddy cultivation.

Keywords: Paddy; Arbuscular Mycorrhizal Fungi (AMF); HCM Mycorrhizal; Biofertilizer; Sustainable Agriculture

References

1. Birla Deep Shikha., et al. "Progress and challenges in improving the nutritional quality of rice (Oryza sativa )”. Critical Reviews in Food Science and Nutrition 57.11 (2017): 2455-2481.
2. Goto Kenta and Bounlouane Douangngeune. "Agricultural modernisation and rural livelihood strategies: the case of rice farming in Laos”. Canadian Journal of Development Studies/Revue canadienne d'études du Développement4 (2017): 467-486.
3. Bwire Denis., et al. "Water management and hydrological characteristics of paddy-rice fields under alternate wetting and drying irrigation practice as climate smart practice: a review”. Agronomy7 (2024): 1421.
4. Kumar Neeraj., et al. "Challenges and opportunities in productivity and sustainability of rice cultivation system: a critical review in Indian perspective”. Cereal Research Communications4 (2022): 573-601.
5. Ejaz , et al. “Economic issues in asian rice wheat cropping systems”. Policy Research Journal 3.4 (2025): 245–259.
6. Paul Ankita and Arti Bhatia. "Exploring the Effects of Contemporary Agricultural Techniques on Soil and Water Pollution in Rice Fields”. Navigating the Nexus: Hydrology, Agriculture, Pollution and Climate Change, Volume 1. Cham: Springer Nature Switzerland (2025): 197-238.
7. Dar Manzoor H., et al. "Abiotic stress tolerance-progress and pathways of sustainable rice production”. Sustainability4 (2021): 2078.
8. Iqbal Junaid., et al. "Sustainable rice production under biotic and abiotic stress challenges”. Sustainable agriculture in the era of the OMICs revolution”. Cham: Springer International Publishing (2023): 241-268.
9. Wahab Abdul., et al. "Role of arbuscular mycorrhizal fungi in regulating growth, enhancing productivity, and potentially influencing ecosystems under abiotic and biotic stresses”. Plants17 (2023): 3102.
10. Khaliq Abdul., et al. "Arbuscular mycorrhizal fungi symbiosis to enhance plant–soil interaction”. Sustainability13 (2022): 7840.
11. Ibrahim Muhammad., et al. "Phosphorus mobilization in plant–soil environments and inspired strategies for managing phosphorus: A review”. Agronomy10 (2022): 2539.
12. Khan Yaseen., et al. "The roles of arbuscular mycorrhizal fungi in influencing plant nutrients, photosynthesis, and metabolites of cereal crops—A review”. Agronomy9 (2022): 2191.
13. Ebbisa Addisu. "Arbuscular mycorrhizal fungi (AMF) in optimizing nutrient bioavailability and reducing agrochemicals for maintaining sustainable agroecosystems. Arbuscular mycorrhizal fungi in agriculture-new insights”. IntechOpen (2022).
14. Chareesri Anupol., et al. "Increased arbuscular mycorrhizal fungal colonization reduces yield loss of rice (Oryza sativa) under drought”. Mycorrhiza 30.2 (2020): 315-328.
15. Zhang Shujuan., et al. "Is resource allocation and grain yield of rice altered by inoculation with arbuscular mycorrhizal fungi?”. Journal of Plant Ecology4 (2015): 436-448.
16. Ruiz-Sánchez Michel., et al. "The arbuscular mycorrhizal symbiosis enhances the photosynthetic efficiency and the antioxidative response of rice plants subjected to drought stress”. Journal of Plant Physiology 11 (2010): 862-869.
17. Mitra Debasis., et al. "Impacts of arbuscular mycorrhizal fungi on rice growth, development, and stress management with a particular emphasis on strigolactone effects on root development”. Communications in Soil Science and Plant Analysis14 (2021): 1591-1621.
18. Suphaphan Saralee., et al. "Promoting Growth Performances and Phytochemicals of Black Upland Rice Through the Co-Inoculation of Arbuscular Mycorrhizal Fungi and Endophytic Fungi Under Drought Conditions”. Journal of Fungi1 (2025): 2.
19. Yadav R S., et al. "Arbuscular mycorrhizal fungi (AMF) for sustainable soil and plant health in salt-affected soils”. Bioremediation of salt affected soils: an Indian perspective. Cham: Springer International Publishing (2017): 133-156.
20. Singh Madhulika., et al. "Unraveling the Synergistic Potential of Mycorrhizal Consortium in Augmenting Salinity Stress Tolerance in Wheat by Advancing Physiological, Metabolic, Nutrient, and Ultrastructural Attributes”. Journal of Soil Science and Plant Nutrition2 (2024): 3714-3733.
21. Zeng Jingyi., et al. “Organic materials and AMF addition promote growth of Taxodium ‘zhongshanshan’ by improving soil structure”. Forests 4 (2023): 731.
22. Tang Haiying., et al. "The critical role of arbuscular mycorrhizal fungi to improve drought tolerance and nitrogen use efficiency in crops”. Frontiers in Plant Science 13 (2022): 919166.
23. Boyno Gökhan., et al. "Synergistic benefits of AMF: development of sustainable plant defense system”. Frontiers in Microbiology 16 (2025): 1551956.
24. Gerdemann J W and Nicolson Y H. “Spores of mycorrhizae Endogone species extracted from soil by wet seiving and decanting”. Transactions of the British Mycological Society 46 (1963): 235-244.
25. Kormanik P and McGraw AC. “Quantification of vesicular arbuscular mycorrhizae in plant roots”. In Methods and Principles of Mycorrhizal Research. Ed. N C Schenck (1984): 29–35.
26. Giovannetti M and Mosse B. “An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots”. New Phytology 84 (1980): 489-500.
27. Gomez Kwanchai A and Arturo A Gomez. “Statistical procedures for agricultural research”. John wiley & sons, (1984).
28. Solaiman M Z and Hiroshi Hirata. "Effect of arbuscular mycorrhizal fungi inoculation of rice seedlings at the nursery stage upon performance in the paddy field and greenhouse”. Plant and Soil1 (1997): 1-12.
29. Yeasmin Tanzima., et al. "Arbuscular mycorrhizal fungus inoculum production in rice plants”. African Journal of Agricultural Research 9 (2007): 463-467.
30. Kalamulla Ruwanthika., et al. "Assessment of community dynamics of arbuscular mycorrhizal fungi in the Rice (Oryza sativa) Rhizosphere and potential application as biofertilizer”. Sustainability 14.24 (2022): 16537.
31. Solaiman Mohammad Zakaria and Hiroshi Hirata. "Effectiveness of arbuscular mycorrhizal colonization at nursery-stage on growth and nutrition in wetland rice (Oryza sativa) after transplanting under different soil fertility and water regimes”. Soil Science and Plant Nutrition 42.3 (1996): 561-571.
32. Mulyadi and Ligeng Jiang. "The combined application of biochar and arbuscular mycorrhizal fungi (AMF) enhanced the physical and chemical properties of soil and rice productivity in Indonesia”. Sustainability12 (2023): 9782.
33. Gomaa M., et al. "Impact of micronutrients and bio-fertilization on yield and quality of rice (Oryza sativa)”. Middle East Journal 4.4 (2015): 919-924.
34. Haifaa MD Iffah and Christopher Moses. "Effect of in vitro produced arbuscular mycorrhizal fungi inoculum on anaerobic direct seeded and transplanted paddy”. Journal of Agricultural Sciences6 (2021): 18.

Citation

Citation: Yogesh Kumar., et al. “Effectiveness of HCM Mycorrhizal (AMF) Inoculation on Growth and Yield Performance of Paddy (Oryza sativa L.)". Acta Scientific Agriculture 10.4 (2026): 03-08.

Copyright

Copyright: © 2026 Yogesh Kumar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.