Effect of Ascorbic Acid on the Cryopreservation of Kenaf (Hibiscus cannabinus L.) Seeds

Olatunji M Olabisi* and Esther E Uchendu

Department of Agronomy, University of Ibadan, Nigeria

*Corresponding Author: Olatunji M Olabisi, Department of Agronomy, University of Ibadan, Nigeria.

Received: June 01, 2022; Published: March 09, 2023

Abstract

Cryopreservation (storage in liquid nitrogen) is a proven method for long-term ex-situ conservation of germplasm. However, the success of cryopreservation depends on the management of oxidative stress imposed on the plant during the cryopreservation procedure. In this study, the role of ascorbic acid (AA) added at the regrowth stage as an antioxidant that enhances the recovery of cryopreserved Kenaf seeds (cv. Ifeken) was examined.

Kenaf seeds were frozen using Plant Vitrification Solutions (PVS) 3 and 4 as the cryoprotectants. The seeds were cultured in the regrowth media supplemented with different concentrations of AA (0.14, 0.28, 0.43, and 0.58 mM) following cryopreservation. The control had no AA in the regrowth medium. Each of the treatments had a sub-control of non-cryopreserved seeds. It was a 2 x 3 factorial experiment laid out in a completely randomized design with three replicates. Effects of AA on survival and germination were determined.

The results showed that there was no significant difference among concentrations of AA on survival and germination of seeds after cryopreservation. A less than 40% survival rate was observed for seeds vitrified using PVS 3. However, for PVS 4, survival increased to a maximum of 63.3% with 0.28 mM AA. Germination of seeds pretreated with PVS 4 generally increased with an increase in AA concentration for both frozen and unfrozen controls.

This is the first report on the cryopreservation of Kenaf seeds and the results showed that AA added at the regrowth stage fairly improved the survival of cryopreserved Kenaf seeds compared to non-AA treatments. Further studies to evaluate other stages of cryopreservation with a wider range of AA concentrations are needed before AA can be recommended for use in the cryopreservation of Kenaf seeds.

Keywords: Ascorbic Acid; Antioxidant; Kenaf Seed; Regrowth; Cryopreservation

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Citation

Citation: Olatunji M Olabisi and Esther E Uchendu. “Effect of Ascorbic Acid on the Cryopreservation of Kenaf (Hibiscus cannabinus L.) Seeds". Acta Scientific Agriculture 7.4 (2023): 43-49.

Copyright

Copyright: © 2023 Olatunji M Olabisi and Esther E Uchendu. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.