Exploration of Markers associated with Heat Stress Tolerance in Pennisetum Glaucum (L.) R. Br.

Sunil Nigombam¹, Reetika Mahajan¹, Avijit Tarafdar¹, Albert Maibam¹, Satish Kumar¹, Monika Dalal¹, SV Amitha CR Mithra¹, Sumer Pal Singh² and Jasdeep Chatrath Padaria¹*

¹ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, India ²ICAR-Division of Genetics, IARI, Pusa Campus, New Delhi, India

*Corresponding Author:Jasdeep Chatrath Padaria, ICAR-National Institute for Plant Biotechnology, Pusa Campus, New Delhi, India.

Received: December 14, 2022; Published: February 20, 2023

Abstract

Development of high-temperature stress-tolerant Pennisetum glaucum (L.) R. Br. genotypes are essential for growing these as an irrigated summer crop for enhanced production. Identification of molecular markers associated with heat stress tolerance is a prerequisite for developing tolerant P. glaucum through molecular breeding strategies. In the present research, 59 expressed sequence tag (EST) derived microsatellite markers [50 EST-Simple Sequence Repeats (SSR) + 9 Sequence Tagged Sites (STS)] were developed from the available heat stress-responsive transcriptome data in pearl millet and validated in 24 diverse genotypes of pearl millet. Among them, four EST-SSRs markers (15.38%) and one STS markers (16.66%) displayed polymorphism respectively. The polymorphic information content (PIC) of newly developed EST-SSRs was in the range from 0.207 to 0.662 with an average value of 0.435. Based on the five polymorphic markers, the 24 pearl millet genotypes were clustered into 5 main and 11 sub-clusters. Annotation of the five transcripts for which polymorphism was detected, was found to code for genes related to stress response and signaling pathway. The EST-SSRs and STS markers thus analyzed would augment the existing SSR marker resource and find their applications in diversity assessment and marker-aided breeding programs for genetic improvement of pearl millet.

Keywords: Pearl Millet; Abiotic Stress Tolerance; SSR Markers; Validation; Polymerase Chain Reaction

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Citation

Citation: Jasdeep Chatrath Padaria., et al. “Exploration of Markers associated with Heat Stress Tolerance in Pennisetum Glaucum (L.) R. Br. Acta Scientific Agriculture 7.3 (2023): 21-31.

Copyright

Copyright: © 2023 Jasdeep Chatrath Padaria., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.