Acta Scientific Agriculture (ASAG)(ISSN: 2581-365X)

Research Article Volume 5 Issue 11

Callus Induction from Leaf and Stem Explants of Selected Turkish Genotypes of Common Bean

Asli Küçükrecep and Dilek Tekdal*

Department of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, Turkey

*Corresponding Author: Dilek Tekdal, Department of Biotechnology, Faculty of Science and Letters, Mersin University, Mersin, Turkey.

Received: September 21, 2021; Published: October 05, 2021



Common bean (Phaseolus vulgaris L.), a species belonging to the family Fabaceae, is one of the most critical legumes consumed and grown globally. Common bean ranks first among grain legumes in terms of cultivation area and production in the world. However, the bean is recalcitrant, making obtaining this plant via in vitro culture techniques a challenge. Due to changing climatic factors and drought conditions that may happen in the future, it is essential to produce plants that make up human food in vitro conditions. With this goal, the effect of different concentrations of 2,4-D and Kinetin and their possible combinations on callus induction in leaf and stem explants of three-bean genotypes (Bitlis117, Göynük, and Önceler) were evaluated. It aimed to determine the best medium that promotes callus formation in different bean genotypes and tissue types. Callogenesis was successfully promoted from leaf and stem explants of all three genotypes tested. The highest callus ratio was obtained from stem explants of Bitlis117 cultured on the MS medium, including 2,4-D (0.5mg L -1) and Kinetin (2.0 mg L -1).

Keywords: In vitro Culture; Leaf; Mersin; Phaseolus vulgaris L.; Stem



  1. Şehirali S. “Yemeklik Tane Baklagiller”. Ankara ÜniversitesiZiraatFakültesiYayınları: DersKitabı: 314, Ankara, (1988).
  2. Elçi, Ş., et al. “Tarlabitkileri”. Ankara ÜniversitesiZiraatFakültesiYayınları: 1385, Ankara (1994).
  3. Delgado-Sanchez., et al. “An organogenic plant regeneration system for common bean (Phaseolus vulgaris )”. Plant Science 170 (2006): 822-827.
  4. Varisai Mohamed., et al. “Organogenesis of Phaseolus angularis: high efficiency of adventitious shoot regeneration from etiolated seedlings in the presence of N6-benzylaminopurine and thidiazuron”. Plant Cell Tissue and Organ Culture 86 (2006): 187-199.
  5. “The State of Food Security and Nutrition in the World 2019” (2019).
  6. Haterlein AJ. “Crop Water Relations”. Wiley (1983): 157-185.
  7. Aragão., et al. “Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris) co-transformed via particle bombardment”. Theoretical and Applied Genetics 93 (1996): 142-150.
  8. Cruz de Carvalho., et al. “Comparison of the physiological responses of Phaseolus and Vigna cultivars when submitted to drought conditions”. Environmental and Experimental Botany 40 (1998): 197-207.
  9. Larkin PJ and WR Scowcroft. “Somaclonal variation-a novel source of variability from cell cultures for plant improvement”. Theoretical Applied Genetics 60 (1981): 197-214.
  10. Evans DA and JE Bravo. “Phenotypic and genotypic stability of tissue culture plants”. Springer, Dordrecht (1986).
  11. Bajaj YPS. Biotechnology in agriculture and forestry II: Somaclonal variation in crop improvement”. Springer-Verlag. Germany (1990).
  12. Velcheva M and Bhowmik G. “Biotechnology as a useful tool in common bean (Phaseolus vulgaris L) improvement”. Euphytica 131 (2003): 189-200.
  13. Hnatuszko-Konka, K. et al. Phaseolus vulgaris”. Recalcitrant Potential 32 (2014): 1205-1215.
  14. Kartha K., et al. “Plant regeneration from meristems of grain legumes: soybean, cowpea, peanut, chickpea, and bean”. Canadian Journal of Botany 59 (1981): 1671-1679.
  15. Mariotti, D., et al. “Genetic transformation of grain legumes: Phaseolus vulgaris and P. coccineus L”. Journal of Genetics and Breeding 43 (1989): 77-82.
  16. McClean P and KF Grafton. “Regeneration of dry bean (Phaseolus vulgaris) via organogenesis”. Plant Science 60 (1989): 117-122.
  17. Franklin CI., et al. “Plant regeneration from seedling explants of green bean (Phaseolus vulgaris) via organogenesis”. Plant Cell Tissue Organ Culture 24 (1991): 199-206.
  18. Malik KA and Saxena PK. “Regeneration in Phaseolus vulgaris: Promotive role of N6-benzylaminopurine in cultures from juvenile leaves”. Planta 184 (1991): 148-150.
  19. Martins IS and Sondahl MR. “Multiple shoot formation from shoot apex cultures of Phaseolus vulgaris L”. Journal of Plant Physiology115 (1984): 205-208.
  20. Zambre MA., et al. “Plant regeneration from embryo-derived callus in Phaseolus vulgaris (common bean) and P. acutifolius A. Gray (tepary bean)”. Plant Cell Reports 17 (1998): 626-630.
  21. Gönülşen N. “Bitki dokukültürleriveuygulamaalanları”. EgeTarımsalAraştırmaEnstitüsü. Yayın No.78 (1987).
  22. Hartmann HT and Kester DE. “Plant Propagation: Principles and Practices”. Englewood Cliffs: Prentice-Hall. (1975).
  23. Murashige T and Skoog F. “A revised medium for rapid growth and bioassays with tobacco tissue cultures”. Physiologia Plantarum 15 (1962): 473-497.
  24. Barwale UB., et al. “Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis”. Planta 167 (1986): 473-481.
  25. Parrott WA., et al. “Effect of genotype on somatic embryogenesis from immature cotyledons of soybean”. Plant Cell Tissue Organ Culture16 (1986): 15-21.


Citation: Asli Küçükrecep and Dilek Tekdal. “Callus Induction from Leaf and Stem Explants of Selected Turkish Genotypes of Common Bean". Acta Scientific Agriculture 5.11 (2021): 02-05.


Acceptance rate32%
Acceptance to publication20-30 days
Impact Factor1.014

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is May 30, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US