Acta Scientific Agriculture (ISSN: 2581-365X)

Research ArticleVolume 5 Issue 8

Genome Editing Technology in Plant Breeding: A Review

Rashmi Regmi1*, Bishnu Bhusal1, Pritika Neupane1, Kushal Bhattarai1, Binju Maharjan1, Suprava Acharya1, Bigyan KC1, Rishav Pandit1, Ram Prashad Mainali2 and Mukti Ram Poudel3

1Department of Plant Breeding, Tribhuwan University, Kathmandu, Bagmati Province, Nepalal
2Technical Officer, Nepal Agricultural Research Council, Khumaltar, Bagmati Province, Nepalal
3Assistant Professor, Department of Plant Breeding, Tribhuwan University, Kathmandu, Bagmati Province, Nepal

*Corresponding Author: Rashmi Regmi, Department of Plant Breeding, Tribhuwan University, Kathmandu, Bagmati Province, Nepal.

Received: March 12, 2021; Published: July 28, 2021

Citation: Rashmi Regmi., et al. “Genome Editing Technology in Plant Breeding: A Review". Acta Scientific Agriculture 5.8 (2021): 41-47.


Global population growth is demanding for more food production in the future. The selection of the plant for cropping has led to the loss of valuable genotypes. Genome-editing technology is the recent advances in plant breeding which allows the insertion, deletion or substitution of the specific loci in the target host cell. Among the homologous recombination (HR) technology, transcription activation-like effector nuclease (TALEN), zinc finger nucleases (ZFN) and clustered regularly interspaced short palindromic repeats (CRISPR); the most popular these days is CRISPR/Cas. Here, we review the methods of genome-editing, their applications, potentials, and the regulatory issues related to genetically modified organisms.

Keywords: Genome-editing; Homologous Recombination; CRISPR/Cas; Indels; Crop Improvements


  1. Araki M and Ishii T. “Towards social acceptance of plant breeding by genome editing”. Trends in Plant Science3 (2015): 145-149.
  2. Hartung F and Schiemann J. “Precise plant breeding using new genome editing techniques: opportunities, safety and regulation in the EU”. The Plant Journal5 (2013): 742-752.
  3. Napier J A., et al. “The challenges of delivering genetically modified crops with nutritional enhancement traits”. Nature Plants (2019): 563-567.
  4. Secretariat of the Convention on Biological Diversity. Cartagena Protocol on Biosafety to the Convention on Biological Diversity: text and annexes. Montreal: Secretariat of the Convention on Biological Diversity (2000).
  5. Maeder L M and Gersbach A C. “Genome -editing Technologies for Gene and Cell Therapy”. Cell Press3 (2016): 430-446.
  6. Gaj T., et al. “ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering”. Trends Biotechnology 31 (2013): 397-405.
  7. Joung K L and Sander D J. “TALENS: a widely applicable technology for targeted genome editing”. Nature Reviews Molecular Cell Biology (2013): 49-55.
  8. Hsu PD., et al. “Development and applications of CRISPR-Cas9 for genome engineering”. Cell 157 (2014): 1262-1278.
  9. Zhang Y., et al. “The emerging and uncultivated potential OF CRISPR technology in plant science”. Nature Plants 5 (2019): 778-794.
  10. Hsu PD., et al. “DNA targeting specificity of RNA-guided Cas9 nucleases”. Nature Biotechnology9 (2013): 827-832.
  11. Lozano-Juste J and Cutler S R. “Plant genome engineering in full bloom”. Trends Plant Science5 (2014): 284-287.
  12. Bhatti A., et al. “Homologous recombination: Additional Information”. Encyclopedia Britannica (2016).
  13. Jung C., et al. “Recent developments in genome editing and applications in plant breeding”. Wiley1 (2017): 1-9.
  14. Li X and Heyer WD. “Homologous recombination in DNA repair and DNA damage tolerance”. Cell Research 18 (2008): 99-113.
  15. Hoeijmakers J. “Genome maintenance mechanisms for preventing cancer”. Nature 411 (2011): 366-374.
  16. Puchta H and Fauser F. “Gene targeting in plants: 25 years later”. The International Journal of Developmental Biology 57 (2013): 629-637.
  17. Urnov DF., et al. “Genome editing with engineered zinc finger nucleases”. Nature Reviews Genetics 11 (2010): 636-646.
  18. Podevin N., et al. “Site‐directed nucleases: a paradigm shift in predictable, knowledge‐based plant breeding”. Trends Biotechnology 31 (2013): 375-383.
  19. Kim M and Kini GA. “Engineering and Application of Zinc Finger Proteins and TALES for Biomedical Research”. Molecules and Cells8 (2017): 533-541.
  20. Chaudhary K., et al. “Transcription activator-like effector nucleases (TALENs): An efficient tool for plant genome editing”. Engineering in Life Sciences4 (2016): 330-337.
  21. Koonin EV., et al. “Diversity, classification and evolution of CRISPR-Cas systems”. Current Opinion in Microbiology 37 (2017): 67-78.
  22. Symington L S. and Gautier J. “Double-strand break end resection and repair pathway choice”. Annual Review of Genetics 45 (2011): 247-271.
  23. Chen K., et al. “CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture”. Annual Review of Plant Biology 70 (2019): 667-697.
  24. Soyk S., et al. “Variation in the flowering gene SELF PRUNING 5G promotes day‐neutrality and early yield in tomato”. Nature Genetics (2017): 162-168.
  25. Klap C., et al. “Tomato facultative parthenocarpy results from SlAGAMOUS‐LIKE 6 loss of function”. Plant Biotechnology Journal5 (2016): 634-647.
  26. Prigge V and Melchinger AE. “Production of haploids and doubled haploids in maize”. Methods in Molecular Biology 877 (2012): 161-172.
  27. Kelliher T., et al. “MATRILINEAL, a sperm‐specific phospholipase, triggers maize haploid induction”. Nature 542 (2017): 105-109.
  28. Zhou H., et al. “Development of commercial thermo‐sensitive genic male sterile rice accelerates hybrid rice breeding using the CRISPR/Cas9‐mediated TMS5 editing system”. Scientific Reports 6 (2016): 37395.
  29. Sauer N J., et al. “Oligonucleotide‐mediated genome editing provides precision and function to engineered nucleases and antibiotics in plants”. Plant Physiology 170 (2016): 1917-1928.
  30. Ricroch A., et al. “Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture”. Emerging Topics in Life Sciences2 (2017): 169-182.
  31. Nemhauser J and Torii K. “Plant synthetic biology for molecular engineering of signalling and development”. Nature Plants 2 (2016): 16010.
  32. Temme K., et al. “Refracting the nitrogen fixation gene cluster from Klebsiella oxytoca”. Proceedings of the National Academy of Sciences of the United States of America18 (2012): 7085-7090.
  33. Jusiak B., et al. “Engineering synthetic gene circuits in living cells with CRISPR technology”. Trends in Biotechnology 7 (2016): 535-547.
  34. Østerberg JT., et al. “Accelerating the domestication of new crops: Feasibility and Approaches”. Trends in Plant Science5 (2017): 373-384.
  35. Li T., et al. “Domestication of wild tomato is accelerated by genome editing”. Nature Biotechnology 36 (2018): 1160-1163.
  36. Gelvin SB. “Plant proteins involved in Agrobacterium-mediated genetic transformation”. Annual Review of Phytopathology 1 (2010): 45-68.
  37. Li, J., et al. “Whole genome sequencing reveals rare off-target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9-edited cotton plants”. Plant Biotechnology Journal 5 (2018): 858-868.
  38. Goodman R E and Tetteh AO. “Suggested improvements for the allergenicity assessment of genetically modified plants used in foods”. Current Allergy and Asthma Report 11 (2011): 317-324.
  39. Prasad KV., et al. “A gain-of-function polymorphism controlling complex traits and fitness in nature”. Science 6098 (2012): 1081-1084.

Copyright: © 2021 Avadhesh Kumar Koshal and Anamika Jain.Rashmi Regmi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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