Dose Dependent Effects of Gamma Radiation on Growth Parameters of Lens culinaris Medikus subsp. Culinaris
Sonia Mejri1*, Imen Hemissi2, Chiheb Brinsi1, Amine Asmi1, Mouldi Saidi1 and Yassine Mabrouk1
1Laboratory of Biotechnology and Nuclear Technologies, National Center for Nuclear Sciences and Technologies, Tunisia
2Institute of Agronomic Research of Tunisia, Avenue Hedi Karray, Tunisia
*Corresponding Author: Sonia Mejri, Laboratory of Biotechnology and Nuclear Technologies, National Center for Nuclear Sciences and Technologies, Tunisia.
June 04, 2021; Published: June 29, 2021
The assessment of treatment dose known as radio sensitivity test is the first step in any breeding program using radiation as physical mutagen. The aim of this preliminary investigation is to assess the dose that results in a 50% reduction of growth (LD50%) that should be enough to obtain lentil mutants for main treatment in a breeding program for resistance to Stemphylium botryosum which is our main goal in the furtherance of our project. Lentil dry seeds were exposed to a 60Co gamma source at doses ranging from 50 - 1000 Gy. For an irradiation dose between 50-400 Gy, results showed a significantly attaint of the growth parameters (P ≤ 0.05) compared to untreated plants. While at doses over than 450 Gy an extremely significant difference (P < 0,001) of plant vegetative growth was recorded. At a dose above 450 Gy, a drastic reduction in the length of roots and shoots as well as in the total content of chlorophyll pigments was recorded. These observations confirm that exposure to lower doses has a simulative effect of physiological parameters, whereas it inhibits and have negative effects of these parameters at higher doses. The obtained results noticed that increased antioxidant capacity and polyphenols content of lentil plants were increased after application of γ-irradiation.
Keywords: Lentil; Gamma Radiation; Germination; Photosynthetic Pigments; Antioxidant Capacity
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