Physiological Interactions of Phaseolus vulgaris L. with Telfairia Mosaic Virus
AAJ Mofunanya*, EA Effa, BA Ngele, FA Akomaye and AO Damian
Department of Botany, Faculty of Biological Sciences, University of Calabar, Cross River State, Nigeria
*Corresponding Author: AAJ Mofunanya, Department of Botany, Faculty of Biological Sciences, University of Calabar, Cross River State, Nigeria.
May 12, 2021; Published: May 26, 2021
The study was conducted to investigate the physiological outcome of interactions of Phaseolus vulgaris L. with Telfairia mosaic virus (TeMV). Seeds of P. vulgaris used in this study were obtained from a farm where the plants were monitored on the field for virus symptoms expression. The seeds were sown, on germination inoculated with the virus at the two leaf stage and allowed to stay for symptom expression. Relative water content, leaf nitrogen content, photosynthetic pigments and enzymes activities were determined at different stages of growth to quantify host virus interactions physiologically. Due to virus infection, some physiological parameters like photosynthetic pigments (chlorophyll a, b and carotenoids), relative water content (RWC), leaf nitrogen content were drastically reduced while all enzymes activity increased significantly. Interaction of P. vulgaris with TeMV led to decreased in RWC and leaf nitrogen content. Percentage decreased in RWC of 40.1% at 12 WAI and 57.6% for leaf nitrogen at 2 WAI was found. Telfairia mosaic virus interaction caused reduction in leaf chlorophyll a content of 53.6%, chlorophyll b (32.9%), chlorophyll a + b (46.6%), ratio of chlorophyll a/b (30.8%) and carotenoids (50.0%). The virus interactions engendered increase in the activities of peroxidase (POD), polyphenol oxidase (PPO), glucose-6-phosphate dehydrogenase (G6PdH), 6-phosphogluconate dehydrogenase (6PGdH) enzymes of 57.0%, 64.9%, 90.4%, 99.1% respectively at 8 WAI. Interactions brought about changes in host physiology with variations in quantity of physiological components affecting whole plant functioning.
Keywords: Enzymes Activities; Interaction; Photosynthetic Pigments; Phaseolus vulgaris
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