Crossability Studies and Characterization by Molecular Markers in Rice (Oryza sativa L.)

Spurty Tripura and VK Khanna*

School of Crop Improvement, College of Post-Graduate Studies, Central Agricultural University, Umiam, Meghalaya, India

*Corresponding Author: VK Khanna, School of Crop Improvement, College of Post-Graduate Studies, Central Agricultural University, Umiam, Meghalaya, India.

Received: May 29, 2018; Published: August 11, 2018

Citation: Spurty Tripura and VK Khanna. “Crossability Studies and Characterization by Molecular Markers in Rice (Oryza sativa L.) Accessions". Acta Scientific Agriculture 2.9 (2018).

Abstract

  Crossability of various accessions of Oryza sativa L. on the basis of pollen development and seed set was studied along with genetic diversity by RAPD and SSR markers. There was little difference in the average pollen fertility among the genotypes but large differences in seed set in crosses. There was a positive correlation between pollen fertility and seed set. Pollen germination in crosses ranged from 25.57 to 55.58 percent. Pollen germination had a positive correlation with seed set only after 10 minutes of pollination. The maximum seed-set was obtained by selfing than in crosses but the pollen tube growth in selfing and crosses did not show much difference. Eleven RAPD and 7 SSR primers were selected (out of 14 and 8, respectively) to assess the genetic diversity of 20 accessions and 2 rice varieties. A total of 42 RAPD amplicons were generated. The value of Jaccard’s similarity coefficient ranged from 0.0 to 0.60 with an average value of 0.280. The polymorphic SSR primers generated a total of 20 alleles, with an average of 2.86 per locus. The value of Jaccard’s similarity coefficient ranged from 0.0 to 0.857 with the average value of 0.308. The correlation coefficient and the significance of the correlation of the matrices based on RAPD and SSR data tested by the Mantel test showed that non-significant correlation (r = 0.18) existed between both matrices.

Keywords: Crossability; Indica Rice; Pollen Tube; Fruit Set; RAPD; SSR

Copyright: © 2018 Spurty Tripura and VK Khanna. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.