The Determination of Sectional and Sub-Generic Relationships among Vicia taxa from Algeria Using Plant Morphology and ISSR Markers

Sakina Bechkri*, Asma Medouri, Mohammed Chekara-Bouziani and Douadi Khelifi

Laboratory of Genetics Biochemistry and Plant Biotechnology, Faculty of Sciences of Nature and Life, University Frères Mentouri Constantine 1, Algeria

*Corresponding Author: Sakina Bechkri, Laboratory of Genetics Biochemistry and Plant Biotechnology, Faculty of Sciences of Nature and Life, University Frères Mentouri Constantine 1, Algeria.

Received: July 11, 2018; Published: August 02, 2018

Citation: Sakina Bechkri., et al. “The Determination of Sectional and Sub-Generic Relationships among Vicia taxa from Algeria Using Plant Morphology and ISSR Markers". Acta Scientific Agriculture 2.9 (2018).

Abstract

  The genetic diversity in 46 germplasms of Vicia L. from Algeria was analyzed using 78 plant morphological traits and 4 ISSR markers. Cluster analysis based on plant morphology revealed two major groups corresponding to the traditional delimitation of the genus. Classification at sectional level also join traditional placement. The results of principal coordinate analysis supported the Unweighted Pair-Group Method using Arithmetic Averages (UPGMA) clustering. These finding support suggestions that V. sativa is an assemblage of taxa presenting an overlap in morphological traits. For the other taxa, the separation is sufficient to warrant distinct sectional status for the taxa studied. Diversity of ISSR profiles has been exploited by UPGMA cluster analysis. Dice and Jaccard indices showed more or less close patterns in the dendrograms. Mantel test gave a strong correlation between the two indices. One hundred and thirteen bands were detected, among these bands, 100% were polymorphic. Primers could be considered suitable for further variability studies in Vicia genus. However, they have some disadvantages which make their usefulness questionable.

Keywords: Algeria; Genetic Diversity; ISSR; Morphology; Vicia

Copyright: © 2018 Sakina Bechkri., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.