Kajal Gandhi and Rajashree Mashru*

Faculty of Pharmacy, The Maharaja Sayajirao University of Baroda, Vadodara, India

*Corresponding Author: Rajashree Mashru, Faculty of Pharmacy, The Maharaja Sayajirao University of Baroda, Vadodara, India.

Received: December 30, 2018; Published: February 24, 2018

Citation: Kajal Gandhi and Rajashree Mashru. “Development and Validation of First Derivative Spectroscopic Method for Simultaneous Estimation of Pyridoxine Hydrochloride with Total and Discrete Citrate in Formulations Used for the Prevention and Recurrence of Nephrolith”. Acta Scientific Pharmaceutical Sciences 2.3 (2018).

Abstract

  Present study deals with the standardization of formulations containing potassium magnesium citrate with Vit.B6. It is used to prevent kidney stones. It is freely available over the counter. So, its estimation becomes important. As it contains inorganic ions as well as Vit.B6, standardization was done using different methods. For estimation of Total Citrate and Vit.B6 simple, rapid and accurate new method is described as Vit.B6 interferes in citrate determination by already reported methods. This method was characterized by excellent precision and accuracy; the coefficient of variation in each case is below the maximal permissible value (%RSD < 2). The assay of the method was in good agreement with standards and enables the method to be adapted for routine analysis of the both substances in combined dosage forms. For estimation of magnesium reported colorimetric method by titan yellow was used as it gives results in acceptance limit ensuring that interference of others does not affect and passes validation. For estimation discrete citrate and potassium ion mathematical back calculation was used as it gave satisfactory results. Hence, proposed methods for standardization of kidney stone formulations were successfully applied on marketed products and found to be simple, rapid and accurate.

Keywords: Pyridoxine Hydrochloride; Total Citrate; Discrete Citrate; Nephrolith Formulations

Copyright: © 2018 Kajal Gandhi and Rajashree Mashru. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.