Molecular Epidemiology and Antimicrobial Resistance of
Campylobacter coli
from Caeca
and Carcass of Poultry in Lebanon
Sukayna M Fadlallah1, Rima El Hajj2, Jeanne El Hage2, Zeina Nasser3, Nada Ghosn
3, Walid Ammar3 and Ghassan M Matar1*
1American University of Beirut, Beirut, Lebanon
2Lebanese Agriculture Research. Institute, Fanar, Lebanon
3Ministry of Public Health, Beirut, Lebanon
*Corresponding Author: Ghassan M Matar, Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine,
American University of Beirut, Beirut, Lebanon.
Received:
February 09, 2018; Published: March 22, 2018
DOI: 10.31080/ASMI.2018.01.0040
Campylobacter
is the primary bacterial cause of human intestinal infections worldwide. Species identification of fifty one
Campylobacter
positive isolates collected at two slaughterhouses in Lebanon was done using the 16 S rRNA sequencing. Antimicrobial
susceptibility testing (AST) was performed using a wide range of β lactam agents and tetracycline. Polymerase chain reactions (PCR)
detection of the
blaOXA-61
and
tet
(O) genes in resistant and susceptible isolates to ampicillin and tetracycline respectively was deter
-
mined. Genomic diversity of the isolates was assessed using Random Amplified Polymorphic DNA (RAPD). Sequencing analysis re
-
vealed that all tested isolates were
Campylobacter coli
. AST showed resistance in the isolates to cephalothin and aztreonam (100%),
cefamandole and cefoxitin (98%), tetracycline (94%), ampicillin (49%), amoxicillin (47%), piperacillin (45%), carbenicillin (37%),
ticarcillin (20%), ceftazidime (18%), cefotaxime (8%) and amoxicillin-clavulanic acid (2%). All ampicillin-resistant isolates and 84%
of the ampicillin-sensitive ones carried the
blaOXA-61
gene. All tetracycline-resistant isolates were positive for the
tet
(O) gene with 98%
encoded on plasmids. RAPD analysis revealed nine distinct clusters with a minimum percentage of 43.5% genomic similarity. This
study emphasizes the importance of surveillance in identifying and controlling commonly circulating food borne pathogens.
Keywords: Campylobacter; Antimicrobial Resistance; Sequencing; Random Amplified Polymorphic DNA (RAPD); Lebanon; Poultry
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