Exploring Anti-
Klebsiella pneumoniae
Activity of Probiotic Lactobacilli of Curd Origin
Shyamapada Mandal* and Debashis Halder
Laboratory of Microbiology and Experimental Medicine, Department of Zoology, University of Gour Banga, Malda, India
*Corresponding Author: Shyamapada Mandal, Profeesor, Laboratory of Microbiology and Experimental Medicine, Department of
Zoology, University of Gour Banga, Malda, West Bengal, India. E-mail:
samtropmed@gmail.com
Received:
February 09, 2018; Published: March 21, 2018
DOI: 10.31080/ASMI.2018.01.0038
The current research evaluates the bacterial growth inhibitory activity of indigenous probiotic lactobacilli and standard
Lacto-
bacillus
strains against multidrug resistant (MDR)
Klebsiella pneumoniae
. The probiotic lactobacilli strains (
Lactobacillus animalis
LMEM6,
L. plantarum
LMEM7,
L. acidophilus
LMEM8 and
L. rhamnosus
LMEM9) from curd samples, and
Lactobacillus fermentum
MTCC 9748 standard strain were tested against indicator bacterial pathogens (n = 2):
K. pneumoniae
B535 clinical and
K. pneumoniae
NTCC 703603 standard strains, following agar overlay and agar-well diffusion techniques. The indicator bacterial strains were tested
as MDR against 6 or more antibiotics with MAR (multiple antibiotic index) 0.4 - 0.73. The ZDI (zone diameter of inhibition) values
from the action of test lactobacilli strains ranged 13.00 ± 1.00 - 33.33 ± 1.53, following agar-overlay method, and 12.00 ± 1.00 - 22.00
± 1.00 mm, following agar-well method, against MDR
K. pneumoniae
indicator strains. The “R” value of lactobacilli strains ranged
3.00 - 13.17 mm, while the bacteriocin activity, in terms of activity unit (AU/ml), ranged 155.60 - 293.33. The study suggests that
the indigenous lactobacilli might play an important role in the protection of host against MDR
K. pneumoniae
infection, and such
probiotic strains may beneficially be employed as biotherapeutic agents in partial replacement or adjunct to antibiotic therapy in the
treatment of MDR
K. pneumoniae
infection.
Keywords: Probiotic Lactobacilli; MDR Klebsiella pneumoniae; Antagonistic Activity; Bacteriocin Activity; MAR Index
-
Al-Madboly LA and Abdullah AK. “Potent antagonistic ac-
tivity of Egyptian Lactobacillus plantarum against multire-
sistant and virulent food-associated pathogens”.
Frontiers
in Microbiology
6 (2015): 347.
-
Halder D.,
et al
. “Indigenous probiotic lactobacillus isolates
presenting antibiotic like activity against human patho-
genic bacteria”.
Biomedicines
5.2 (2017): E31.
-
Paczosa MK.,
et al
. “Klebsiella pneumoniae: going on the
offense with a strong defense”.
Microbiology and Molecular
Biology Reviews
80.3 (2016): 629-661.
-
Lebeaux D.,
et al
. “Biofilm-related infections: bridging the
gap between clinical management and fundamental as-
pects of recalcitrance toward antibiotics”.
Microbiology
and Molecular Biology Reviews
78.3 (2014): 510-543.
-
Halder D and Mandal S. “Antibacterial potentiality of com-
mercially available probiotic lactobacilli and curd lacto-
bacilli strains, alone and in combination, against human
pathogenic bacteria”.
Translational Biomedicine
7 (2016):
2.
-
Halder D and Mandal S. “Curd lactobacilli with probiotic
potentiality”.
Translational Biomedicine
6.2 (2015): 1-6.
-
Lagrafeuille R.,
et al
. “Opposing effect of Lactobacillus on
in vitro Klebsiella pneumoniae in biofilm and in an in vivo
intestinal colonisation model”.
Beneficial Microbes
9.1
(2017): 87-100.
-
Al-Mathkhury.,
et al
. “Inhibitory effect of lactobacilli fil
-
trate on Klebsiella pneumoniae Biofilm”.
The Iraqi Post
-
graduate Medical Journal
11.1 (2012): 168-179.
-
Clinical and Laboratory Standards Institute (CLSI): Per
-
formance standards for antimicrobial susceptibility test-
ing (2011) 21st
informational supplement M100S21. CLSI,
Wayne, Pa (2011).
-
Nandi S and Mandal S. “Bacteriological profiling of com
-
mercially available eye cosmetics and their antibiotic sus-
ceptibility pattern”.
Translational Biomedicine
7.3 (2016):
1-8.
-
Shokryazdan P.,
et al
. “Probiotic potential of Lactobacillus
strains with antimicrobial activity against some human
pathogenic strains”.
BioMed Research International
(2014).
-
Carasi P.,
et al
. “Safety characterization and antimicrobial
properties of kefir-isolated Lactobacillus kefiri”.
BioMed
Research International
(2014): 208974.
-
Pisano MB.,
et al
. “Preliminary evaluation of probiotic
properties of Lactobacillus strains isolated from Sardinian dairy products”.
BioMed Research International
(2014):
286390.
-
Iyapparaj P.,
et al
. “Optimization of bacteriocin produc-
tion by Lactobacillus sp. MSU3IR against shrimp bacterial
pathogens”.
Aquatic Biosystems
9.1 (2013): 12.
-
Yu WL and Chuang YC. “Clinical features, diagnosis, and
treatment of Klebsiella pneumoniae infection” (2018).
-
Lu CH.,
et al
. “Klebsiella pneumoniae meningitis: analysis on
clinical features of thirty-two adult patients”.
Chinese Medi-
cal Journal
60.6 (1997): 296-302.
-
Lin WP.,
et al
. “The Antimicrobial Susceptibility of Klebsiella
pneumoniae from community settings in Taiwan, a trend
analysis”.
Scientific Reports
6 (2016): 36280.
-
Gupta N.,
et al
. “Carbapenem-resistant Enterobacteriaceae:
Epidemiology and prevention”.
Clinical Infectious Diseases
53.1 (2011): 60-67.
-
Collado MC.,
et al
. “The impact of probiotic on gut health”.
Current Drug Metabolism
10.1 (2009): 68-78.
-
Corr SC.,
et al
. “Understanding the mechanisms by which
probiotics inhibit gastrointestinal pathogens”.
Advance Food
and Nutrition Research
56 (2009): 1-15.
-
Sanchez B.,
et al
. “Extracellular proteins secreted by probi-
otic bacteria as mediators of effects that promote mucosa-
bacteria interactions”.
Microbiology
156.11 (2010) 3232-
3242.
-
Vuotto C.,
et al
. “Probiotics to counteract biofilm-associated
infections: promising and conflicting data”.
International
Journal of Oral Science
6.4 (2014) 189-194.
-
Saranya S and Hemashenpagam N. “Antagonistic activity
and antibiotic sensitivity of Lactic acid bacteria from fer
-
mented dairy products”.
Advances in Applied Science Re-
search
2.4 (2011): 528-534.
-
Kormin S.,
et al
. “Bacteriocin-producing lactic acid bacteria
isolated from traditional fermented food”.
Malaysian Jour
-
nal of Medical Sciences
8.1(2001): 63-68.
-
Shehata MG.,
et al
. “Screening of isolated potential probiotic
lactic acid bacteria for cholesterol lowering property and
bile salt hydrolase activity”.
Annals of Agricultural Sciences
61.1 (2016): 65-75.